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Haw Yang

Research Focus


The properties of a given molecule or nanoscale structure are conventionally characterized by its mean value that is averaged over a great number of molecules (N~1023). Such an ensemble-averaged description becomes inadequate, however, as the number of molecules under consideration decreases where the observable range of fluctuation approximately scales with N-1/2. To an observer in the macroscopic world, the changes in the microscopic states as a function of time and location are no longer deterministic but probabilistic. The central premise of the current research thrust is that these local stochastic fluctuations give rise to the chemical and physical transformations as they appear in the macroscopic world. This general problem is studied at the most fundamental, single-molecule and single-particle level. New experimental approaches are devised to enable real-time observations of individual molecules as they move and function. Theoretical frameworks are concurrently formulated to afford a quantitative understanding. Current research topics include: (1) High-resolution quantitative single-molecule protein dynamics, where we focus on large-amplitude conformational transitions in proteins and attempt to develop a predictive understanding of these molecular machines. (2) Real-time 3D single-particle tracking spectroscopy, where we are developing new techniques to follow the reactivity of a molecule-scale probe in real time and to directly correlate the chemical or physical processes with the 3D spatial location of the probe.

Selected Recent Publications

  • “Change-Point Localization and Wavelet Spectral Analysis of Single-Molecule Time Series,” H. Yang, to appear in Single Molecule Biophysics: Theories and Experiments, a special volume in Advances in Chemical Physics, edited by Tamiki Komatsuzaki, Haw Yang, and Robert Silbey, with targeted publication year 2010.
     
  • “Conformational studies of protein dynamics using single-molecule FRET,” J. A. Hanson, Y.-W. Tan, and H. Yang, in Single Particle Tracking and Single Molecule Energy Transfer: Applications in the Bio and Nano Sciences edited by Christoph Bräuchle, Don Lamb and Jens Michaelis, Wiley-VCH, in press with targeted publication year 2009.
     
  • “A New Quantification of Local Transition Heterogeneity of Multiscale Complex Networks Reconstructed from Single-Molecule Time Series,” C.-B. Li, H. Yang, and T. Komatsuzaki, J. Phys. Chem., in press (2009).
     
  • “Microfluidic infrared microscopy for real-time chemical imaging of biofilms,” H.-Y. N. Holman, R. Miles, Z. Hao, E. Wozei, L. M. Anderson, and H. Yang, Anal. Chem., in press (2009).
     
  • "Real-time three-dimensional single-particle tracking spectroscopy for complex systems,” D. Montiel and H. Yang, invited review for Laser and Photons Review, in press (2009).
     
  • “Bipolar Cellular Morphology of Malignant Melanoma in Unstained Human Melenoma Skin Tissue,” K. Zhang, W.-K. Zhang, C.-Y. Yang, and H. Yang, J. Biomed. Opt., 14, 024042 (2009).
     
  • “Direct Mg2+ binding activates Adenylate Kinase from Escherichia coli,” Y.-W. Tan, J. A. Hanson, and H. Yang, J. Biol. Chem., 284, 3306 (2009).
     
  • “Quantitative Evaluation of Cross-Correlation between Two Finite-Length Time Series with Applications to Single-Molecule FRET Experiments,” J. A. Hanson and H. Yang, J. Phys. Chem. B, 112, 13962-13970 (2008).
     
  • “Observation of correlated emission intensity and polarization fluctuations in single CdSe/ZnS quantum dots,” D. Montiel and H. Yang, J. Phys. Chem. A, 112, 9352-9355 (2008).
     
  • “Model-Free Statistical Reduction of Single-Molecule Time Series,” H. Yang, in Theory and Evaluation of Single-Molecule Signals edited by E. Barkai, F. Brown, M. Orrit, and H. Yang, World Scientific Publishing, October (2008).
     
  • “A Simplified Model for Lysogenic Regulation through DNA looping,” L. M. Anderson and H. Yang, Proceedings of The 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, pp 607–610, Vancouver, Canada, Aug. 20-24, (2008).
     
  • “Detection and Characterization of Dynamical Heterogeneity in an Event Series Using Wavelet Correlation,” H. Yang, J. Chem. Phys., 129, 074701 (2008). This work was selected by the Virtual Journal of Biological Physics Research, 16, Aug. 15 (2008), appearing in the SINGLE MOLECULE DYNAMICS section.
     
  • “Observation of Spectral Anisotropy of Gold Nanoparticles by Single-Particle Tracking Spectroscopy,” H. Cang, D. Montiel, C. S. Xu, and H. Yang, J. Chem. Phys. 129, 044503 (2008).
     
  • “A General Statistical Test for Correlations in a Finite-Length Time Series,” J. A. Hanson and H. Yang, J. Chem. Phys., 128, 214101 (2008).
     
  • “Progress in Single-Molecule Tracking Spectroscopy,” H. Cang, C. S. Xu, and H. Yang, Chem. Phys. Lett., 457, 285-291 (2008) (invited review for the FRONTIER ARTICLE SERIES). (Cover)
     
  • “Joint Statistical Analysis of Multi-Channel Time Series from Single Quantum Dot-(Cy5)n Constructs,” C. S. Xu, H. Kim, C. C. Hayden, and H. Yang, J. Phys. Chem. B, 112, 5917-5923 (2008).
     
  • “New role for DNA looping in phage ,” L. M. Anderson and H. Yang, Proc. Natl. Acad. Sci. USA, 105, 5827-5832 (2008).
     
  • “Complex Network of Protein Conformation Fluctuations in Single-Molecule Time Series,” C.-B. Li, H. Yang, and T. Komatsuzaki, Proc. Natl. Acad. Sci. USA, 105, 536-541 (2008).
     
  • “Illuminating the Mechanistic Roles of Enzyme Conformational Dynamics,” J. A. Hanson, K. Duderstadt, L. P. Watkins, S. Bhattacharyya, J. Brokaw, J.-W. Chu, and H. Yang, Proc. Natl. Acad. Sci. USA, 104, 18055-18060 (2007).
     
  • “Single Quantum Dots as Local Temperature Markers,” S. Li, K. Zhang, J.-M. Yang, L. Lin, and H. Yang, Nano Lett. 7, 3102-3105 (2007). This work was highlighted in Technology Research News, and also in the November issue of Nanophotonics (2007).
     
  • “Guiding a Confocal Microscope by Single Fluorescent Nanoparticles,” H. Cang, C. S. Xu, D. Montiel, and H. Yang, Opt. Lett., 32, 2729-2731 (2007). This work was highlighted in the October issue of Nature Photonics, 1, 561 (2007).
     
  • “Multi-parameter Fluorescence Spectroscopy of Single Quantum Dot-Dye FRET Hybrids,” C. S. Xu, H. Kim, H. Yang, and C. C. Hayden, J. Am. Chem. Soc., 129, 11008-11009 (2007). This work was highlighted in the December issue of Biophotonics, 14, 41 (2007).
     
  • “DNA Transformation via Local Heat Shock,” S. Li, L. M. Anderson, J.-M. Yang, L. Lin, and H. Yang, Appl. Phys. Lett., 91, 013902 (2007). This work was selected by the Virtual Journal of Biological Physics Research, 14, Jul. 15 (2007), appearing in the DNA CONFORMATIONAL DYNAMICS section.
     
  • “Single-Particle Light Scattering: Imaging and Dynamical Fluctuations in the Polarization and Spectral Response,” H. Yang, J. Phys. Chem. A, 111, 4987–4997 (2007).
     
  • “MEMS Temperature Characterization by CdSe Quantum Dots,” S. Li, K. Zhang, J.-M. Yang, L. Lin, and H. Yang, The 14th International Conference on Solid-State Sensors, Actuators and Microsystems, pp 1369-1372, Lyon, France, June 10-14, 2007.
     
  • “Rapid and Quantitative Sizing of Nanoparticles Using Three-Dimensional Single-Particle Tracking,” C. S. Xu, H. Cang, D. Montiel, and H. Yang, J. Phys. Chem. C, 111, 32-35 (2007).
     
  • “DNA Transformation by Local Heat Shock,” S. Li, L. M. Anderson, L. Lin, and H. Yang, Proceedings of the 20th IEEE Micro Electro Mechanical Systems Conference, pp 533–536. Kobe, Japan, January 2007.
     
  • “Optically Active Sum Frequency Generation Microscopy for Cellular Imaging,” K. Zhang, N. Ji, Y. R. Shen, and H. Yang, Ultrafast Phenomena XV, Eds. P. Corkum, D. Jonas, D. Miller, A. M. Weiner, (Springer-Verlag, Berlin Heidelberg, 2007) p. 825.
     
  • “Quantitative Characterization of Changes in Dynamical Behavior for Single-Particle Tracking Studies,” D. Montiel, H. Cang, and H. Yang, J. Phys. Chem. B, 110, 19763-19770 (2006).
     
  • “Confocal Three Dimensional Tracking of A Single Nanoparticle with Concurrent Spectroscopic Readouts,” H. Cang, C. M. Wong, C. S. Xu, A. H. Rizvi, and H. Yang, Appl. Phys. Lett., 88, 223901 (2006). This work was selected by the Virtual Journal of Nanoscale Science and Technology, 13, Jun. 12 (2006), appearing in the IMAGING SCIENCE AND TECHNOLOGY section. See also the news brief, Analytical Currents, in Analytical Chemistry, Anal. Chem., 78, 5238 (2006).
     
  • “Continuous Distribution of Emission States from Single CdSe/ZnS Quantum Dots,” K. Zhang, H. Chang, A. Fu, A.P. Alivisatos, and H. Yang, Nano Letters, 6, 843–847 (2006).
     
  • “Quantitative Single-Molecule Conformational Distributions: A Case Study with Poly-(L-proline),” L.P. Watkins, H. Chang, and H. Yang, J. Phys. Chem. A, 110, 5191–5203 (2006).
     
  • “Three-Dimensional Chiral Imaging by Sum Frequency Generation,” N. Ji, K. Zhang, H. Yang, and Y.R. Shen, J. Am. Chem. Soc.128, 3482–3483 (2006).
     
  • “Photon-by-Photon Determination of Emission Bursts from Diffusing Single Chromophores,” K. Zhang and H. Yang, J. Phys. Chem. B, 109, 21930–21937 (2005).
     
  • “Detection of Intensity Change Points in Time-Resolved Single-Molecule Measurements,” L. P. Watkins and H. Yang, J. Phys. Chem. B, 109, 617–628 (2005).
     
  • “Discrete Nanostructures of Quantum Dots/Au with DNA,” A. Fu, C. M. Micheel, J. Cha, H. Chang, H. Yang, and A. P. Alivisatos, J. Am. Chem. Soc., 126, 10832-10833 (2004).
     
  • “Information Bounds and Optimal Analysis of Dynamic Single Molecule Measurements,” L. P. Watkins and H. Yang, Biophys. J. 86, 4015-4029 (2004).
     

Haw Yang

Yang Lab Webpage
hawyang@princeton.edu
Frick Laboratory, 225
Phone: 609-258-3578

Faculty Assistant:
Clarice Gethers
cgethers@princeton.edu
Frick Laboratory, 221
Phone: 609-258-0355