• Mating
• Sporulation, Tetrad Dissection
• Colony PCR
• PCR test for HAP1+ (wt) vs. S288c (TY insertion) allele
• DNA prep (M. Hickman)
• Protein/Western (M. Hickman)
• Yeast DNA content by FACS

• Probe labeling and FISH (Singer/coverslip hyb)
• In-solution hyb (Singer or Stellaris probes)
• Imaging with Olympus scope

• RNA Prep (pdf)
• Affymetrix Tiling Arrays 6/11 (pdf)
• Scott's RNA Label/Wash/Scan 8/2010
• Agilent Yeast cDNA 8x15k(pdf)
• All Array Protocols (pdf for 2-side printing)

                      8x15k worksheet (xls)

                      4x44k worksheet (xls)

• Botlab Chemostat Manual (pdf)
• Evolutions (pdf)
• FACS Analysis of Competitions (pdf)
• Pulse Experiments (pdf)
• YMC Synchrony

• Chemostat Manual 2005
• More Yeast, Genotype, chemostat and other protocols

FISH, Chemostat and other methods

• Vectorette PCR (html)
• Mulholland's EM (pdf)

For other old protocols email sandy at genomics dot princeton dot edu