From Mark Hickman

Repaired HAP1 allele in S288C strain

The sequence of the repaired HAP1 allele is identical to that of S288C (SGD version) if the Ty sequence is removed. Sequencing was performed from 224 bp upstream of start codon to 554 bp downstream of stop codon.

There are two sequences below:

1. S288C allele from SGD
2. Repaired HAP1 allele

PCR to distinguish wild-type and S288C HAP1 alleles

There are two separate PCR reactions for the two alleles. The antisense primer is the same for the two reactions. However, the two sense primers are not compatible so the reactions must be carried out separately. These primers work well for colony PCR.

(1) S288C allele

Sense CTTTATCAACAATGGAATCCC (in Ty) (primer FO560)

Antisense ATCAGCTTACGGAATGTTAC (460 bp downstream of HAP1 stop codon) (primer MH39 or FO2641)

583 bp product

(2) wild-type allele

Sense GGGAGCTTTACCATCTTTAGATAGG (510 bp upstream of HAP1 stop codon) (primer FO1498)

Antisense ATCAGCTTACGGAATGTTAC (460 bp downstream of HAP1 stop codon) (primer MH39 or FO2641)

970 bp product

hap1 mutant genomic sequence (S288C, from SGD)

ATG and TAG of coding sequence are in bold (these are according to SGD annotation of the sequence).
The actual 3’ end of the HAP1 coding sequence is in red text. (The sequence between the bolded TAG and the red text is the Ty insertion.)

GCTTCTGTCACTTAGTAAGCAAATGTACGAAACCCAGAGCATTGTATGTACACCAGCAGTCGTTTTTTGTAGCTCTTTTCCATGGATGGACGATGATGGCAAGACGCGATCGTGGCGGAGCCTCACTAAGAAAAACAATTGTGGAGAATCTATACACACAGAAATCGTACTTCAAGAAATATTGATTAGCGAGCCTTTAAGCACAGAACAATCTCTATAGCCAACATCTTGTGGAAGCTACAACTGTTTTTCTTGTTCCCGCTTGAGTAGGGCCTTACAAGGCAAGACCGTATAAAATAAAGATAACTCCTCCGCCGTATCCTGAATTTCGTGTGCCCTTTCTCTCTCTCTCTCTTCCTCTTTCTTCCTTTCCCTTTCCCTCTTCTTCTTATCTCAACAACCGCCCAGCGCATTAGCAAACCACAGTACGAGCTGTACGGCTGAGCCACTGCACCTGTCTGGCTTGCCCCTTTTTGTTGAGTAACCGGGTAATAATTCGGCTGTCTAAACGATACAATCCTCGTTCCACCCACACCCATTCACCCGTCGGGCGAAATCCACCCTCGCGGCCTCGTTTAGAATACCAGGCAACGGCACCAGCAACGGCCCCGGTAACTCGATCGCGTTCATATATATCGGATATACTCTTGAGGCACAATTTTTTTCGGTTTTCGGTTTTTTCTATTATTCTTCGAGATGCAAAGCACACATTTGAGACTCACAACTCAAGATACCGCAAGCACACAGACGCTATTGTTCTCCCAAAGAATAGGTTCCTGCAGTGCTCTTTTAAGGTTTGAAGATATTGCACAAAATTGTATCTGATTTCATCTCATCGAACCTCATTCCATCAACTTCCTTTTATCAAAGCATCTTGTTTTGTTTTCGCAGAAAATTTTTCTTCCCTTTCAATTTGGCCTTTTAGTTTTTCTCCAGTCGCAGGCAAGAAGGTAAGGAAATAGAAGAAAAAGAAAAAAAAAAAAAGGGAACAATAGGTTAGATGTCGAATACCCCTTATAATTCATCTGTGCCTTCCATTGCATCCATGACCCAGTCTTCGGTCTCAAGAAGTCCTAACATGCATACAGCAACTACGCCCGGTGCCAACACCAGCTCTAACTCTCCACCCTTGCACATGTCTTCAGATTCGTCCAAGATCAAGAGGAAGCGTAACAGAATTCCGCTCAGTTGCACCATTTGTCGGAAAAGGAAAGTCAAATGTGACAAACTCAGACCACACTGCCAGCAGTGCACTAAAACTGGGGTAGCCCATCTCTGCCACTACATGGAACAGACCTGGGCAGAAGAGGCAGAGAAAGAATTGCTGAAGGACAACGAATTAAAGAAGCTTAGGGAGCGCGTAAAATCTTTAGAAAAGACTCTTTCTAAGGTGCACTCTTCTCCTTCGTCTAACTCCTTGAAAAGTTACAACACTCCCGAGAGCAGCAACCTGTTTATGGGTAGCGATGAACACACCACCCTTGTTAATGCAAATACAGGCTCTGCTTCCTCTGCCTCGCATATGCATCAGCAACAACAGCAACAGCAGCAACAGGAACAACAACAAGACTTTTCCAGAAGTGCGAACGCCAACGCGAATTCCTCGTCCCTTTCTATCTCAAATAAATATGACAACGATGAGCTGGACTTAACTAAGGACTTTGATCTTTTGCATATCAAAAGTAACGGAACCATCCACTTAGGTGCCACCCACTGGTTGTCTATCATGAAAGGTGACCCGTACCTAAAACTTTTGTGGGGTCATATCTTCGCTATGAGGGAAAAGTTAAATGAATGGTACTACCAAAAAAATTCGTACTCTAAGCTGAAGTCAAGCAAATGTCCCATCAATCACGCGCAAGCGCCGCCTTCTGCCGCTGCCGCCGCTACCAGAAAATGTCCTGTTGATCACTCCGCGTTTTCGTCTGGCATGGTGGCCCCAAAGGAGGAGACTCCTCTTCCTAGGAAATGTCCAGTTGACCACACCATGTTCTCTTCGGGAATGATTCCTCCCAGAGAGGACACTTCGTCCCAGAAGAGGTGTCCCGTTGACCACACCATGTATTCCGCAGGAATGATGCCGCCCAAGGACGAGACACCTTCCCCATTTTCCACTAAAGCTATGATAGACCATAACAAGCATACAATGAATCCGCCTCAGTCAAAATGTCCTGTGGACCATAGAAACTATATGAAGGATTATCCCTCTGACATGGCAAATTCTTCTTCGAACCCGGCAAGTCGTTGCCCCATTGACCATTCAAGCATGAAAAATACAGCGGCCTTACCAGCTTCAACGCACAATACCATCCCACACCACCAACCACAGTCCGGATCTCATGCTCGTTCGCATCCCGCACAAAGCAGGAAACATGATTCCTACATGACAGAATCTGAAGTCCTCGCAACACTTTGTGAGATGTTGCCACCAAAGCGCGTCATCGCATTATTCATCGAGAAATTCTTCAAACATTTATACCCTGCCATTCCAATCTTAGATGAACAGAATTTCAAAAATCACGTGAATCAAATGCTTTCGTTGTCTTCGATGAATCCCACAGTTAACAACTTTGGTATGAGCATGCCATCTTCATCTACACTAGAGAACCAACCCATAACACAAATCAATCTTCCAAAACTTTCCGATTCTTGTAACTTAGGTATTCTGATAATAATCTTGAGATTGACATGGCTATCCATACCTTCTAATTCCTGCGAAGTCGACCTGGGAGAAGAAAGTGGCTCATTTTTAGTGCCCAACGAATCTAGCAATATGTCTGCATCTGCATTGACCTCGATGGCTAAAGAAGAATCACTTCTGCTAAAGCATGAGACACCGGTCGAGGCACTGGAGCTATGTCAAAAATACTTGATTAAATTCGATGAACTTTCTAGTATTTCCAATAACAACGTTAATTTAACCACGGTGCAGTTTGCCATTTTTTACAACTTCTATATGAAAAGTGCCTCTAATGATTTGACTACCTTGACAAATACCAACAACACTGGCATGGCCAATCCTGGTCACGATTCCGAGTCTCACCAGATCCTATTGTCCAATATTACTCAAATGGCCTTTAGTTGTGGGTTACACAGAGACCCTGATAATTTTCCTCAATTAAACGCTACCATTCCAGCAACCAGCCAGGACGTGTCTAACAACGGGAGCAAAAAGGCAAACCCTAGCACCAATCCAACTTTGAATAACAACATGTCTGCTGCCACTACCAACAGCAGTAGCAGATCTGGCAGTGCTGATTCAAGAAGTGGTTCTAACCCTGTGAACAAGAAGGAAAATCAGGTTAGTATCGAAAGATTTAAACACACTTGGAGGAAAATTTGGTATTACATTGTTAGCATGGATGTTAACCAATCTCTTTCCCTGGGGAGCCCTCGACTACTAAGAAATCTGAGGGATTTCAGCGATACAAAGCTACCAAGTGCGTCAAGGATTGATTATGTTCGCGATATCAAAGAGTTAATCATTGTGAAGAATTTTACTCTTTTTTTCCAAATTGATTTGTGTATTATTGCTGTATTAAATCACATTTTGAATGTTTCTTTAGCAAGAAGCGTGAGAAAATTTGAACTGGATTCATTGATTAATTTATTGAAAAATCTGACCTATGGTACTGAGAATGTCAATGATGTAGTGAGCTCCTTGATCAACAAAGGGTTATTACCAACTTCGGAAGGTGGTTCTGTAGATTCAAATAATGATGAAATTTACGGTCTACCGAAACTACCCGATATTCTAAACCATGGTCAACATAACCAAAACTTGTATGCTGATGGAAGAAATACTTCTAGTAGTGATATAGATAAGAAATTGGACCTTCCTCACGAATCTACAACGAGAGCTCTATTCTTTTCCAAGCATATGACAATTAGAATGTTGCTGTACTTATTGAACTACATTTTGTTTACTCATTATGAACCAATGGGCAGTGAAGATCCTGGTACTAATATCCTAGCTAAGGAGTACGCTCAAGAGGCATTAAATTTTGCCATGGATGGCTACAGAAACTGCATGATTTTCTTCAACAATATCAGAAACACCAATTCACTATTCGATTACATGAATGTTATCTTGTCTTACCCTTGTTTGGACATTGGACATCGTTCTTTACAATTTATCGTTTGTTTGATCCTGAGAGCTAAATGTGGCCCATTGACTGGTATGCGTGAATCATCGATCATTACTAATGGTACATCAAGTGGATTTAATAGTTCGGTAGAAGATGAGGACGTCAAAGTTAAACAAGAATCTTCTGATGAATTGAAAAAAGACGATTTCATGAAAGATGTAAATTTGGATTCAGGCGATTCATTAGCAGAGATTCTAATGTCAAGAATGCTGCTATTTCAAAAACTAACAAAACAACTATCAAAGAAGTACAACTACGCTATTCGTATGAACAAATCCACTGGATTCTTTGTCTCTTTACTAGATACACCTTCAAAGAAATCAGACTCGAAATCGGGTGGTAGTTCATTCATGTTGGGTAATTGGAAACATCCAAAGGTTTCAAACATGAGCGGATTTCTTGCTGGTGACAAAGACCAATTACAGAAATGCCCCGTGTACCAAGATGCGCTGGGGTTTGTTAGTCCAACCGGTGCTAATGAAGGTTCTGCTCCGATGCAAGGCATGTCCTTACAGGGCTCTACTGCTAGGATGGGAGGGACCCAGTTGCCACCAATTAGATCATACAAACCTATCACGTACACAAGTAGTAATCTACGTCGTATGAATGAAACGGGTGAGGCAGAAGCTAAGAGAAGAAGATTTAATGATGGCTATATTGATAATAATAGTAACAACGATATACCTAGAGGAATCAGCCCAAAACCTTCAAATGGGCTATCATCGGTGCAGCCACTATTATCGTCATTTTCCATGAACCAGCTAAACGGGGGTACCATTCCAACGGTTCCATCGTTAACCAACATTACTTCACAAATGGGAGCTTTACCATCTTTAGATAGGATCACCACTAATCAAATAAATTTGCCAGACCCATCTAGAGATGAAGCATTTGACAACTCCATCAAGCAAATGACGCCTATGACAAGTGCATTCATGAATGCTAATACTACAATTCCAAGTTCAACTTTAAACGGGAATATGAACATGAATGGAGCTGGAACTGCGAATACAGATACAAGTGCCAACGGCAGTGCTTTATCGACACTGACAAGCCCACAAGGCTCAGACTTAGCATCCAATTCTGCTACACAGTATAAACCTGACTTAGAAGACTTTTTGATGCAAAATTCTAACTTTAATGGGCTAATGATAAATCCTTCCAGTCTGGTAGAAGTTGTTGGTGGATACAACGATCCTAATAACCTTGGAAGAAATGACGCGGTTGATTTTCTACCCGTTGATAATGTTGAAATTGATGGTGTTGGAATAAAAATCAACTATCATCTACTAACTAGTATTTACGTTACTAGTATATTATCATATACGGTGTTAGAAGATGACGCAAATGATGAGAAATAGTCATCTAAATTAGTGGAAGCTGAAACGCAAGGATTGATAATGTAATAGGATCAATGAATATTAACATATAAAACGATGATAATAATATTTATAGAATTGTGTAGAATTGCAGATTCCCTTTTATGGATTCCTAAATCCTCGAGGAGAACTTCTAGTATATTCTGTATACCTAATATTATAGCCTTTATCAACAATGGAATCCCAACAATTATCTCAACACCCACACATTTCTCATGGTAGCGCCTGTGCTTCGGTTACTTCTAAGGAAGTCCACACAAATCAAGATCCGTTAGACGTTTCAGCTTCCAAAACAGAAGAATGTGAGAAGGCTTCCACTAAGGCTAACTCTCAACAGACAACAACACCTGCTTCATCAGCTGTTCCAGAGAACCCCCATCATGCCTCTCCTCAACCTGCTTCAGTACCACCTCCACAGAATGGGCCGTACCCACAGCAGTGCATGATGACCCAAAACCAAGCCAATCCATCTGGTTGGTCATTTTACGGACACCCATCTATGATTCCGTATACACCTTATCAAATGTCGCCTATGTACTTTCCACCTGGGCCACAATCACAGTTTCCGCAGTATCCATCATCAGTTGGAACGCCTCTGAGCACTCCATCACCTGAGTCAGGTAATACATTTACTGATTCATCCTCAGCGGACTCTGATATGACATCCACTAAAAAATATGTCAGACCACCACCAATGTTAACCTCACCTAATGACTTTCCAAATTGGGTTAAAACATACATCAAATTTTTACAAAACTCGAATCTCGGTGGTATTATTCCGACAGTAAACGGAAAACCCGTACGTCAGATCACTGATGATGAACTCACCTTCTTGTATAACACTTTTCAAATATTTGCTCCCTCTCAATTCCTACCTACCTGGGTCAAAGACATCCTATCCGTTGATTATACGGATATCATGAAAATTCTTTCCAAAAGTATTGAAAAAATGCAATCTGATACCCAAGAGGCAAACGACATTGTGACCCTGGCAAATTTGCAATATAATGGCAGTACACCTGCAGATGCATTTGAAACAAAAGTCACAAACATTATCGACAGACTGAACAATAATGGCATTCATATCAATAACAAGGTCGCATGCCAATTAATTATGAGAGGTCTATCTGGCGAATATAAATTTTTACGCTACACACGTCATCGACATCTAAATATGACAGTCGCTGAACTGTTCTTAGATATCCATGCTATTTATGAAGAACAACAGGGATCGAGAAACAGCAAACCTAATTACAGGAGAAATCTGAGTGATGAGAAGAATGATTCTCGCAGCTATACGAATACAACCAAACCCAAAGTTATAGCTCGGAATCCTCAAAAAACAAATAATTCGAAATCGAAAACAGCCAGGGCTCACAATGTATCCACATCTAATAACTCTCCCAGCACGGACAACGATTCCATCAGTAAATCAACTACTGAACCGATTCAATTGAACAATAAGCACGACCTTCACCTTAGGCCAGGAACTTACTGAATCTACGGTAAATCACACTAATCATTCTGATGATGAACTCCCTGGACACCTCCTTCTCGATTCAGGAGCATCACGAACCCTTATAAGATCTGCTCACCACATACACTCAGCATCATCTAATCCTGACATAAACGTAGTTGATGCTCAAAAAAGAAATATACCAATTAACGCTATTGGTGACCTACAATTTCACTTCCAGGACAACACCAAAACATCAATAAAGGTATTGCACACTCCTAACATAGCCTATGACTTACTCAGTTTGAATGAATTGGCTGCAGTAGATATCACAGCATGCTTTACCAAAAACGTCTTAGAACGGTCTGACGGCACTGTACTTGCACCTATCGTAAAATATGGAGACTTTTACTGGGTATCTAAAAAGTACTTGCTTCCATCAAATATCTCCGTACCCACCATCAATAATGTCCATACAAGTGAAAGTACACGCAAATATCCTTATCCTTTCATTCATCGAATGCTTGCGCATGCCAATGCACAGACAATTCGATACTCACTTAAAAATAACACCATCACGTATTTTAACGAATCAGATGTCGACTGGTCTAGTGCTATTGACTATCAATGTCCTGATTGTTTAATCGGCAAAAGCACCAAACACAGACATATCAAAGGTTCACGACTAAAATACCAAAATTCATACGAACCCTTTCAATACCTACATACTGACATATTTGGTCCAGTTCACAACCTACCAAATAGTGCACCATCCTATTTCATCTCATTTACTGATGAGACAACAAAATTCCGTTGGGTTTATCCATTACACGACCGTCGCGAGGACTCTATCCTCGATGTTTTTACTACGATACTAGCTTTTATTAAAAACCAGTTTCAGGCCAGTGTCTTAGTTATACAAATGGACCGTGGTTCTGAGTATACTAACAGAACTCTCCATAAATTCCTTGAAAAAAAAATGGTATAACTCCATGCTATACAACCACAGCGGATTCCCGAGCACATGGAGTCGCTGAACGGCTAAACCGTACCTTATTAGATGACTGCCGTACTCAACTGCAATGTAGTGGTTTACCGAACCATTTATGGTTCTCTGCAATCGAATTTTCTACTATTGTGAGAAATTCACTAGCTTCACCTAAAAGCAAAAAATCTGCAAGACAACATGCTGGCTTGGCAGGACTTGATATCAGTACTTTGTTACCTTTCGGTCAACCTGTTATCGTCAATGATCACAACCCTAACTCCAAAATACATCCTCGTGGCATCCCAGGCTACGCTCTACATCCGTCTCGAAACTCTTATGGATATATCATCTATCTTCCGTCCTTAAAGAAGACAGTAGATACAACTAACTATGTTATTCTTCAGGGCAAGGAATCCAGATTAGATCAATTCAATTACGACGCACTCACTTTCGATGAAGACTTAAACCGTTTAACTGCTTCATATCATTCGTTCATTGCGTCAAATGAGATCCAAGAATCCAATGATCTTAACATAGAATCTGACCATGACTTCCAATCCGACATTGAACTACATCCTGAGCAACCGAGAAATGTCCTTTCAAAAGCTGTGAGTCCAACCGATTCCACACCTCCGTCAACTCATACTGAAGATTCGAAACGTGTTTCTAAAACCAATATTCGCGCACCCAGAGAAGTTGACCCCAACATATCTGAATCTAATATTCTTCCATCAAAGAAGAGATCTAGCACCCCCCAAATTTCCAATATCGAGAGTACCGGTTCGGGTGGTATGCATAAATTAAATGTTCCTTTACTTGCTCCCATGTCCCAATCTAACACACATGAGTCGTCGCACGCCAGTAAATCTAAAGATTTCAGACACTCAGACTCGTACAGTGAAAATGAGACTAATCATACAAACGTACCAATATCCAGTACGGGTGGTACCAACAACAAAACTGTTCCGCAGATAAGTGACCAAGAGACTGAGAAAAGGATTATACACCGTTCACCTTCAATCGATGCTTCTCCACCGGAAAATAATTCATCGCACAATATTGTTCCTATCAAAACGCCAACTACTGTTTCTGAACAGAATACCGAGGAATCTATCATCGCTGATCTCCCACTCCCTGATCTACCTCCAGAATCTCCTACCGAATTCCCTGACCCATTTAAAGAACTCCCACCGATAAATTCTCGTCAAACTAATTCCAGTTTGGGTGGTATTGGTGACTCTAATGCCTATACTACTATCAACAGTAAGAAAAGATCATTAGAAGATAATGAAACTGAAATTAAGGTATCACGAGACACATGGAATACTAAGAATATGCGTAGTTTAGAACCTCCGAGATCGAAGAAACGAATTCACCTGATTGCAGCTGTAAAAGCAGTAAAATCAATCAAACCAATACGGACAACCTTACGATACGATGAGGCAATCACCTATAATAAAGATATTAAAGAAAAAGAAAAATATATCGAGGCATACCACAAAGAAGTCAATCAACTGTTGAAGATGAAAACTTGGGACACTGACGAATATTATGACAGAAAAGAAATAGACCCTAAAAGAGTAATAAACTCAATGTTTATCTTCAACAAGAAACGTGACGGTACTCATAAAGCTAGATTTGTTGCAAGAGGTGATATTCAGCATCCTGACACTTACGACTCAGGCATGCAATCCAATACCGTACATCACTATGCATTAATGACATCCCTGTCACTTGCATTAGACAATAACTACTATATTACACAATTAGACATATCTTCGGCATATTTGTATGCAGACATCAAAGAAGAATTATACATAAGACCTCCACCACATTTAGGAATGAATGATAAGTTGATACGTTTGAAGAAATCACTTTATGGATTGAAACAAAGTGGAGCGAACTGGTACGAAACTATCAAATCATACCTGATACAACAATGTGGTATGGAAGAAGTTCGTGGATGGTCATGCGTATTTAAAAACAGTCAAGTGACAATTTGTTTATTCGTAGATGATATGGTATTGTTTAGCAAAAATCTAAATTCAAACAAAAGAATTATAGAGAAGCTTAAGATGCAATACGACACCAAGATTATAAATCTAGGCGAAAGTGATGAGGAAATTCAATATGACATACTTGGCTTAGAAATCAAATATCAAAGAGGTAAATACATGAAATTAGGTATGGAAAACTCATTAACTGAGAAAATACCCAAATTAAACGTACCTTTGAATCCAAAAGGAAGAAAACTTAGCGCTCCAGGTCAACCAGGTCTTTATATAGACCAGGATGAACTAGAAATAGATGAAGATGAATACAAAGAGAAGGTACATGAAATGCAAAAGTTGATTGGTCTAGCTTCATATGTTGGATATAAATTTAGATTTGACTTACTATACTACATCAACACACTTGCTCAACATATACTATTCCCCTCTAGGCAAGTTTTAGACATGACATATGAGTTAATACAATTCATGTGGGACACTAGAGATAAACAATTAATATGGCACAAAAACAAACCTACCAAGCCAGATAATAAACTAGTCGCAATAAGCGATGCTTCATATGGTAACCAACCATATTACAAGTCACAAATTGGTAACATTTTCCTACTCAACGGAAAAGTGATTGGAGGAAAGTCGACAAAGGCTTCGTTAACATGCACTTCAACTACAGAAGCAGAAATACACGCAGTCAGTGAAGCTATACCGCTATTGAATAACCTCAGTCACCTTGTGCAAGAACTTAACAAGAAACCAATTATTAAAGGCTTACTTACTGATAGTAGATCAACGATCAGTATAATTAAGTCTACAAATGAAGAGAAATTTAGAAACAGATTTTTTGGCACAAAGGCAATGAGACTTAGAGATGAAGTATCAGGTAATAATTTATACGTATACTACATCGAGACCAAGAAGAACATTGCTGATGTGATGACAAAACCTCTTCCGATAAAAACATTTAAACTATTAACAAACAAATGGATTCATTAGATCTATTACATTATGGGTGGTATGTTGGAATAAAAATCAACTATCATCTACTAACTAGTATTTACGTTACTAGTATATTATCATATACGGTGTTAGAAGATGACGCAAATGATGAGAAATAGTCATCTAAATTAGTGGAAGCTGAAACGCAAGGATTGATAATGTAATAGGATCAATGAATATTAACATATAAAACGATGATAATAATATTTATAGAATTGTGTAGAATTGCAGATTCCCTTTTATGGATTCCTAAATCCTCGAGGAGAACTTCTAGTATATTCTGTATACCTAATATTATAGCCTTTATCAACAATGGAATCCCAACAATTATCTAATTACCCACAAATTTCTCAGATGGACTAGTAGATTTTTATAGAGCAGATTTTCCAATATGGGAGTGATGCGGTTTGTTTATGTATAAATAGAAAGGGACATATTTTTATTATTATTATTTTAGTTATTTAGCATGTATGTTATTTTATCGGTGAATTGTAATGATCGAATAATAATTGGAGGTTGAAGAAGTTGCAAAAAATATTTACTTGGACGAGTCCGGAATCGAACCGGAGACCTCTCCCATGCTAAGGGAGCGCGCTACCGACTACGCCACACGCCCAATTTTCTTGTTGTCTGTTGTGTTTCAGAATGGGGTACGTCAGTATGATAATAATCATCCTAAACGTTCTTAAATTACATATGAAACAACCTTATAGCAAAACGAACAGAATGAGCAACATGAGATGAAACTCCGCCTCCTTAACTGAACTTTCCAAACGTATAAACGCCTGAAAAATTAGTTTAGATCCGAGATTCCGCGCTTCCACCATCAAGTATGATCCATATTTTATATAATATATAAGATAAGTAACATTCCGTAAGCTGATAATCCCTTTTGGCAACTCGTTACTTCCCAAAGACTGTTTATATTAGGATTGTCAAGGGACCCCGGTATTGCTCGAGCCCGTAATACAACAGTTGTCTTTCACTGCTCTCTATGTTTTGAGACCTCCAGTTCACCACATATATTCGATCTTTTTCTTACTGTTGAAGCAAGAATCTGTTGGAATGAAGGCCTAGTATTGTCTATTGACTAATATTCATCTTACTAGTATATTATCATATACAGTGTAAGAAGATGGCATAAAGATTGAGAACCAGTCATCAGACTAATGAAAGCTGAAATGCAAGGACTGATAATGTAATGGGATAATGAAATATATAAAACGGAAGGAGAAATAACCGTGATATTATTATGTAGATTTACCGACTCCACTTGGAGGATTTCTCTATTCTCGAGGGAAACTTGGTATATTCGTACACCTAATATTATAGCTCTTGATAACAATGAAATTT

HAP1 genomic sequence (in repaired S288C HAP1 strain)

GCTTCTGTCACTTAGTAAGCAAATGTACGAAACCCAGAGCATTGTATGTACACCAGCAGTCGTTTTTTGTAGCTCTTTTCCATGGATGGACGATGATGGCAAGACGCGATCGTGGCGGAGCCTCACTAAGAAAAACAATTGTGGAGAATCTATACACACAGAAATCGTACTTCAAGAAATATTGATTAGCGAGCCTTTAAGCACAGAACAATCTCTATAGCCAACATCTTGTGGAAGCTACAACTGTTTTTCTTGTTCCCGCTTGAGTAGGGCCTTACAAGGCAAGACCGTATAAAATAAAGATAACTCCTCCGCCGTATCCTGAATTTCGTGTGCCCTTTCTCTCTCTCTCTCTTCCTCTTTCTTCCTTTCCCTTTCCCTCTTCTTCTTATCTCAACAACCGCCCAGCGCATTAGCAAACCACAGTACGAGCTGTACGGCTGAGCCACTGCACCTGTCTGGCTTGCCCCTTTTTGTTGAGTAACCGGGTAATAATTCGGCTGTCTAAACGATACAATCCTCGTTCCACCCACACCCATTCACCCGTCGGGCGAAATCCACCCTCGCGGCCTCGTTTAGAATACCAGGCAACGGCACCAGCAACGGCCCCGGTAACTCGATCGCGTTCATATATATCGGATATACTCTTGAGGCACAATTTTTTTCGGTTTTCGGTTTTTTCTATTATTCTTCGAGATGCAAAGCACACATTTGAGACTCACAACTCAAGATACCGCAAGCACACAGACGCTATTGTTCTCCCAAAGAATAGGTTCCTGCAGTGCTCTTTTAAGGTTTGAAGATATTGCACAAAATTGTATCTGATTTCATCTCATCGAACCTCATTCCATCAACTTCCTTTTATCAAAGCATCTTGTTTTGTTTTCGCAGAAAATTTTTCTTCCCTTTCAATTTGGCCTTTTAGTTTTTCTCCAGTCGCAGGCAAGAAGGTAAGGAAATAGAAGAAAAAGAAAAAAAAAAAAAGGGAACAATAGGTTAGATGTCGAATACCCCTTATAATTCATCTGTGCCTTCCATTGCATCCATGACCCAGTCTTCGGTCTCAAGAAGTCCTAACATGCATACAGCAACTACGCCCGGTGCCAACACCAGCTCTAACTCTCCACCCTTGCACATGTCTTCAGATTCGTCCAAGATCAAGAGGAAGCGTAACAGAATTCCGCTCAGTTGCACCATTTGTCGGAAAAGGAAAGTCAAATGTGACAAACTCAGACCACACTGCCAGCAGTGCACTAAAACTGGGGTAGCCCATCTCTGCCACTACATGGAACAGACCTGGGCAGAAGAGGCAGAGAAAGAATTGCTGAAGGACAACGAATTAAAGAAGCTTAGGGAGCGCGTAAAATCTTTAGAAAAGACTCTTTCTAAGGTGCACTCTTCTCCTTCGTCTAACTCCTTGAAAAGTTACAACACTCCCGAGAGCAGCAACCTGTTTATGGGTAGCGATGAACACACCACCCTTGTTAATGCAAATACAGGCTCTGCTTCCTCTGCCTCGCATATGCATCAGCAACAACAGCAACAGCAGCAACAGGAACAACAACAAGACTTTTCCAGAAGTGCGAACGCCAACGCGAATTCCTCGTCCCTTTCTATCTCAAATAAATATGACAACGATGAGCTGGACTTAACTAAGGACTTTGATCTTTTGCATATCAAAAGTAACGGAACCATCCACTTAGGTGCCACCCACTGGTTGTCTATCATGAAAGGTGACCCGTACCTAAAACTTTTGTGGGGTCATATCTTCGCTATGAGGGAAAAGTTAAATGAATGGTACTACCAAAAAAATTCGTACTCTAAGCTGAAGTCAAGCAAATGTCCCATCAATCACGCGCAAGCGCCGCCTTCTGCCGCTGCCGCCGCTACCAGAAAATGTCCTGTTGATCACTCCGCGTTTTCGTCTGGCATGGTGGCCCCAAAGGAGGAGACTCCTCTTCCTAGGAAATGTCCAGTTGACCACACCATGTTCTCTTCGGGAATGATTCCTCCCAGAGAGGACACTTCGTCCCAGAAGAGGTGTCCCGTTGACCACACCATGTATTCCGCAGGAATGATGCCGCCCAAGGACGAGACACCTTCCCCATTTTCCACTAAAGCTATGATAGACCATAACAAGCATACAATGAATCCGCCTCAGTCAAAATGTCCTGTGGACCATAGAAACTATATGAAGGATTATCCCTCTGACATGGCAAATTCTTCTTCGAACCCGGCAAGTCGTTGCCCCATTGACCATTCAAGCATGAAAAATACAGCGGCCTTACCAGCTTCAACGCACAATACCATCCCACACCACCAACCACAGTCCGGATCTCATGCTCGTTCGCATCCCGCACAAAGCAGGAAACATGATTCCTACATGACAGAATCTGAAGTCCTCGCAACACTTTGTGAGATGTTGCCACCAAAGCGCGTCATCGCATTATTCATCGAGAAATTCTTCAAACATTTATACCCTGCCATTCCAATCTTAGATGAACAGAATTTCAAAAATCACGTGAATCAAATGCTTTCGTTGTCTTCGATGAATCCCACAGTTAACAACTTTGGTATGAGCATGCCATCTTCATCTACACTAGAGAACCAACCCATAACACAAATCAATCTTCCAAAACTTTCCGATTCTTGTAACTTAGGTATTCTGATAATAATCTTGAGATTGACATGGCTATCCATACCTTCTAATTCCTGCGAAGTCGACCTGGGAGAAGAAAGTGGCTCATTTTTAGTGCCCAACGAATCTAGCAATATGTCTGCATCTGCATTGACCTCGATGGCTAAAGAAGAATCACTTCTGCTAAAGCATGAGACACCGGTCGAGGCACTGGAGCTATGTCAAAAATACTTGATTAAATTCGATGAACTTTCTAGTATTTCCAATAACAACGTTAATTTAACCACGGTGCAGTTTGCCATTTTTTACAACTTCTATATGAAAAGTGCCTCTAATGATTTGACTACCTTGACAAATACCAACAACACTGGCATGGCCAATCCTGGTCACGATTCCGAGTCTCACCAGATCCTATTGTCCAATATTACTCAAATGGCCTTTAGTTGTGGGTTACACAGAGACCCTGATAATTTTCCTCAATTAAACGCTACCATTCCAGCAACCAGCCAGGACGTGTCTAACAACGGGAGCAAAAAGGCAAACCCTAGCACCAATCCAACTTTGAATAACAACATGTCTGCTGCCACTACCAACAGCAGTAGCAGATCTGGCAGTGCTGATTCAAGAAGTGGTTCTAACCCTGTGAACAAGAAGGAAAATCAGGTTAGTATCGAAAGATTTAAACACACTTGGAGGAAAATTTGGTATTACATTGTTAGCATGGATGTTAACCAATCTCTTTCCCTGGGGAGCCCTCGACTACTAAGAAATCTGAGGGATTTCAGCGATACAAAGCTACCAAGTGCGTCAAGGATTGATTATGTTCGCGATATCAAAGAGTTAATCATTGTGAAGAATTTTACTCTTTTTTTCCAAATTGATTTGTGTATTATTGCTGTATTAAATCACATTTTGAATGTTTCTTTAGCAAGAAGCGTGAGAAAATTTGAACTGGATTCATTGATTAATTTATTGAAAAATCTGACCTATGGTACTGAGAATGTCAATGATGTAGTGAGCTCCTTGATCAACAAAGGGTTATTACCAACTTCGGAAGGTGGTTCTGTAGATTCAAATAATGATGAAATTTACGGTCTACCGAAACTACCCGATATTCTAAACCATGGTCAACATAACCAAAACTTGTATGCTGATGGAAGAAATACTTCTAGTAGTGATATAGATAAGAAATTGGACCTTCCTCACGAATCTACAACGAGAGCTCTATTCTTTTCCAAGCATATGACAATTAGAATGTTGCTGTACTTATTGAACTACATTTTGTTTACTCATTATGAACCAATGGGCAGTGAAGATCCTGGTACTAATATCCTAGCTAAGGAGTACGCTCAAGAGGCATTAAATTTTGCCATGGATGGCTACAGAAACTGCATGATTTTCTTCAACAATATCAGAAACACCAATTCACTATTCGATTACATGAATGTTATCTTGTCTTACCCTTGTTTGGACATTGGACATCGTTCTTTACAATTTATCGTTTGTTTGATCCTGAGAGCTAAATGTGGCCCATTGACTGGTATGCGTGAATCATCGATCATTACTAATGGTACATCAAGTGGATTTAATAGTTCGGTAGAAGATGAGGACGTCAAAGTTAAACAAGAATCTTCTGATGAATTGAAAAAAGACGATTTCATGAAAGATGTAAATTTGGATTCAGGCGATTCATTAGCAGAGATTCTAATGTCAAGAATGCTGCTATTTCAAAAACTAACAAAACAACTATCAAAGAAGTACAACTACGCTATTCGTATGAACAAATCCACTGGATTCTTTGTCTCTTTACTAGATACACCTTCAAAGAAATCAGACTCGAAATCGGGTGGTAGTTCATTCATGTTGGGTAATTGGAAACATCCAAAGGTTTCAAACATGAGCGGATTTCTTGCTGGTGACAAAGACCAATTACAGAAATGCCCCGTGTACCAAGATGCGCTGGGGTTTGTTAGTCCAACCGGTGCTAATGAAGGTTCTGCTCCGATGCAAGGCATGTCCTTACAGGGCTCTACTGCTAGGATGGGAGGGACCCAGTTGCCACCAATTAGATCATACAAACCTATCACGTACACAAGTAGTAATCTACGTCGTATGAATGAAACGGGTGAGGCAGAAGCTAAGAGAAGAAGATTTAATGATGGCTATATTGATAATAATAGTAACAACGATATACCTAGAGGAATCAGCCCAAAACCTTCAAATGGGCTATCATCGGTGCAGCCACTATTATCGTCATTTTCCATGAACCAGCTAAACGGGGGTACCATTCCAACGGTTCCATCGTTAACCAACATTACTTCACAAATGGGAGCTTTACCATCTTTAGATAGGATCACCACTAATCAAATAAATTTGCCAGACCCATCTAGAGATGAAGCATTTGACAACTCCATCAAGCAAATGACGCCTATGACAAGTGCATTCATGAATGCTAATACTACAATTCCAAGTTCAACTTTAAACGGGAATATGAACATGAATGGAGCTGGAACTGCGAATACAGATACAAGTGCCAACGGCAGTGCTTTATCGACACTGACAAGCCCACAAGGCTCAGACTTAGCATCCAATTCTGCTACACAGTATAAACCTGACTTAGAAGACTTTTTGATGCAAAATTCTAACTTTAATGGGCTAATGATAAATCCTTCCAGTCTGGTAGAAGTTGTTGGTGGATACAACGATCCTAATAACCTTGGAAGAAATGACGCGGTTGATTTTCTACCCGTTGATAATGTTGAAATTGATGGACTAGTAGATTTTTATAGAGCAGATTTTCCAATATGGGAGTGATGCGGTTTGTTTATGTATAAATAGAAAGGGACATATTTTTATTATTATTATTTTAGTTATTTAGCATGTATGTTATTTTATCGGTGAATTGTAATGATCGAATAATAATTGGAGGTTGAAGAAGTTGCAAAAAATATTTACTTGGACGAGTCCGGAATCGAACCGGAGACCTCTCCCATGCTAAGGGAGCGCGCTACCGACTACGCCACACGCCCAATTTTCTTGTTGTCTGTTGTGTTTCAGAATGGGGTACGTCAGTATGATAATAATCATCCTAAACGTTCTTAAATTACATATGAAACAACCTTATAGCAAAACGAACAGAATGAGCAACATGAGATGAAACTCCGCCTCCTTAACTGAACTTTCCAAACGTATAAACGCCTGAAAAATTAGTTTAGATCCGAGATTCCGCGCTTCCACCATCAAGTATGATCCATATTTTATATAATATATAAGATAAGTAACATTCCGTAAGCTGATAATCCCTTTTGGCAACTCGTTACTTCCCAAAGACTGTTTATATTAGGATTGTCAAGGGACCCCGGTATTGCTCGAGCCCGTAATACAACAGTTGTCTTTCACTGCTCTCTATGTTTTGAGACCTCCAGTTCACCACATATATTCGATCTTTTTCTTACTGTTGAAGCAAGAATCTGTTGGAATGAAGGCCTAGTATTGTCTATTGACTAATATTCATCTTACTAGTATATTATCATATACAGTGTAAGAAGATGGCATAAAGATTGAGAACCAGTCATCAGACTAATGAAAGCTGAAATGCAAGGACTGATAATGTAATGGGATAATGAAATATATAAAACGGAAGGAGAAATAACCGTGATATTATTATGTAGATTTACCGACTCCACTTGGAGGATTTCTCTATTCTCGAGGGAAACTTGGTATATTCGTACACCTAATATTATAGCTCTTGATAACAATGAAATTT