Gloria Brar, UCSF, Using high-resolution translation measurements to define meiotic cellular remodeling and redefine genome coding
Meiosis is a complex and well-conserved program of cellular differentiation. I performed genome-wide measurements of mRNA abundances and new translation using ribosome profiling through yeast meiosis to better understand the molecular basis for the full cellular restructuring that accompanies meiotic chromosome segregation. This global view of translation through a developmental process revealed great complexity to the protein complement of these differentiating cells, both in the number and the structure of expressed genes. Nearly every gene in the yeast genome was translated in meiotic cells in a strongly stage-specific manner, including disparate and regulated induction of conserved canonical stress pathways such as the Unfolded Protein Response. Translational regulation contributed broadly to this temporal control of protein synthesis timing through several distinct mechanisms. Additionally, meiotic translation of thousands of novel short ORFs was observed, expanding our view of what constitutes a coding region even in the most well annotated eukaryotic genome.
Location: Lewis Thomas Lab 003
Date/Time: 12/07/12 at 09:30 am - 12/07/12 at 10:30 pm
Category: Special Seminars
Department: Lewis-Sigler Institute