Light Sheet Microscopy
In Light Sheet fluorescence microscopy the samples are illuminated from the side by a thin sheet of light positioned exactly at the focal plane of the objective, and images are gathered by a camera. This approach reduces considerably the out-of-focus fluorescence compared to epi-fluorescence, therefore improving the contrast, and reducing photobleaching. The method is fast as it does not rely on beam scanning and is readily compatible with large field of view.
This imaging approach finds its niche with thick and relatively transparent (low scattering) samples.
In the facility, we have available two set-ups for Light Sheet Microscopy.