Events - Daily
|Monday, April 09|
Johan Elf, Uppsala University, Transcription factor search kinetics at the level of single molecules
Probing transcription factor sliding in living cells
Transcription factors are believed to combine 1-dimensional (1D) sliding on DNA with 3D diffusion in the cytoplasm to find their specific binding sites fast and accurately. We have developed a single molecule assay that allows us to study the sliding process in living bacteria. We find that the lac repressor slides 45±10 base pairs on chromosomal DNA. Furthermore, DNA-bound proteins near the operator block sliding both into and out from the operator. This implies that TF kinetics can be modulated by proteins not interacting with the TF as long as they bind DNA within its sliding distance.
Joseph Henry Room, Jadwin Hall · 12:00 p.m.– 1:00 p.m.
David Sabatini, MIT/Whitehead Institute, Growth control through the mTOR pathway
mTOR is the target of the immunosuppressive drug rapamycin and the central component of a nutrient- and hormone-sensitive signaling pathway that regulates cell growth and proliferation. We now appreciate that this pathway becomes deregulated in many human cancers and has an important role in the control of metabolism and aging. We have identified two distinct mTOR-containing proteins complexes, one of which regulates growth through S6K and another that regulates cell survival through Akt. These complexes, mTORC1 and mTORC2, define both rapamycin-sensitive and insensitive branches of the mTOR pathway. I will discuss new results from our lab on the regulation and functions of the mTORC1 and mTORC2 pathways.
Carl Icahn Lab 101 · 4:15 p.m.– 5:15 p.m.