Events - Daily
November 05, 2012 >>
|Monday, November 05|
Marcelo Magnasco, Rockefeller, title tba
Carl Icahn Lab 101 · 12:00 p.m.– 1:00 p.m.
L. Stirling Churchman, Harvard Med. Sch., Visualizing transcription with single nucleotide resolution using nascent transcript sequencing
It is now clear that transcription is more complicated than what was thought even a few years ago both in the intricate use of post-initiation control and the mass production of rapidly degraded transcripts. Here we present an approach, native elongating transcript sequencing - NET-seq, that provides a quantitative measure of RNAP density genome-wide with single nucleotide precision. Application of NET-seq in Saccharomyces cerevisiae reveals pervasive polymerase pausing and backtracking and the widespread production of unstable transcripts.
Carl Icahn Lab 101 · 4:15 p.m.– 5:15 p.m.