Events - Weekly
|Sunday, November 23|
|Monday, November 24|
Roy Bar-Ziv, Weizmann/Harvard, "Programmable on-chip DNA compartments as “Artificial cells”"
We will discuss the assembly of two-dimensional DNA compartments fabricated in silicon as artificial cells capable of metabolism, programmable protein synthesis, and communication. Metabolism is maintained by continuous diffusion of nutrients and products through a thin capillary, connecting protein synthesis in the DNA compartment with the environment. We programmed protein expression cycles, autoregulated protein levels, and a signaling expression gradient, equivalent to a morphogen, in an array of interconnected compartments at the scale of an embryo. Gene expression in the DNA compartment reveals a rich, dynamic system that is controlled by geometry, offering a means for studying biological networks outside a living cell.
Joseph Henry Room, Jadwin Hall · 12:00 p.m.– 1:00 p.m.
John Murray, University of Pennsylvania, "Decoding development through the C. elegans lineage"
Our lab aims to understand how genomes encode diverse gene expression patterns and cellular phenotypes during animal development. We use C. elegans to study developmental gene expression regulation because its transparency and invariant development allow automated quantitative analysis of gene expression by microscopy. We have developed imaging and computational methods for automated cell tracking in C. elegans embryos and used these methods to define the wild-type expression patterns for over 100 transcription factors at single cell resolution. Even this small number of genes can distinguish most cells in the embryo. We continue to develop these tools with the goal of extending this information to the full genome.
Mining the expression data has led to several novel results. We are asking how context regulates the response of cells to signaling pathways such as the Wnt pathway, and have identified a novel transmitotic memory mechanism that may explain some of the context-dependence of these targets. Combining coexpression data with cell tracking in mutant embryos has identified novel redundant regulators of progenitor identity in early embryos. Together, our work sheds light on how C. elegans embryos are able to develop with such a high degree of precision and identifies regulatory mechanisms and factors likely to be conserved across animals.
Carl Icahn Lab 101 · 4:15 p.m.– 5:15 p.m.
|Tuesday, November 25|
|Wednesday, November 26|
|Thursday, November 27|
|Friday, November 28|
|Saturday, November 29|