L. Stirling Churchman, Harvard Med. Sch., Visualizing transcription with single nucleotide resolution using nascent transcript sequencing
It is now clear that transcription is more complicated than what was thought even a few years ago both in the intricate use of post-initiation control and the mass production of rapidly degraded transcripts. Here we present an approach, native elongating transcript sequencing - NET-seq, that provides a quantitative measure of RNAP density genome-wide with single nucleotide precision. Application of NET-seq in Saccharomyces cerevisiae reveals pervasive polymerase pausing and backtracking and the widespread production of unstable transcripts.
Location: Carl Icahn Lab 101
Date/Time: 11/05/12 at 4:15 pm - 11/05/12 at 5:15 pm
Category: Quantitative & Computational Biology
Department: Lewis-Sigler Institute