Joerg Bewersdorf, Yale
The diffraction limit of light has constrained the resolution of light microscopes in the far field since its discovery more than a century ago. Structures smaller than about half the wavelength of light could therefore not be resolved by light microscopes. The realization that this limit can be broken has triggered a revolution in imaging, especially in biological applications which heavily depend on light microscopy. By taking advantage of optically switching fluorescent molecules on and off, 25 nm spatial resolution or better, more than 10-fold better than in conventional microscopy, is now achievable .
In my presentation, I will provide an overview of the different approaches that are currently developed and applied. I will provide examples of current developments in my group in STED microscopy and PALM/STORM, two of the most prominent nanoscopy techniques [2-4].
Disclaimer: J.B. declares financial interest in Vutara Inc., a start-up company producing nanoscopes.
1.T.J. Gould, S.T. Hess, and J. Bewersdorf (2012) “Optical Nanoscopy: from Acquisition to Analysis”, Annu. Rev. Biomed. Eng. 14:231–254
2.T.J. Gould, J.R. Myers, and J. Bewersdorf (2011) “Total internal reflection STED microscopy”, Opt. Express 19(14): 13351–13357.
3.T.J. Gould, D. Burke, J. Bewersdorf*, M.J. Booth* (2012) “Adaptive Optics Enables 3D STED Microscopy in Aberrating Specimens”, Opt. Express 20(19):20998-21009.
4.F. Huang, T.M.P. Hartwich, F.E. Rivera-Molina, Y. Lin, W.C. Duim, J.J. Long, P.D. Uchil, J.R. Myers, M.A. Baird, W. Mothes, M.W. Davidson, D. Toomre, J. Bewersdorf (2013) “Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms”, Nature Methods 10(7): 653-658.
Joerg Bewersdorf earned his doctoral degree at the Max Planck Institute for Biophysical Chemistry in Goettingen, Germany, in 2002 under the mentorship of Dr. Stefan Hell, a pioneer in super-resolution microscopy. After a 4-year research appointment at The Jackson Laboratory in Maine, USA, he has joined Yale University School of Medicine as an Assistant Professor.
His research focuses on the development and application of new fluorescence microscopy techniques for biomedical research. Over the last 14 years, he has made significant contributions to the development of multiphoton microscopy (multifocal multiphoton microscope) and different fields of super-resolution microscopy (4Pi microscopy, PALM/STORM, STED microscopy). Dr. Bewersdorf has served as the Co-chair of the Nanoscale Biophysics Subgroup of the Biophysical Society of America and is co-founder of a start-up company developing super-resolution microscopes.
Location: Carl Icahn Lab 101
Date/Time: 11/11/13 at 4:15 pm - 11/11/13 at 5:15 pm
Category: Quantitative & Computational Biology
Department: Lewis-Sigler Institute