Hari Shroff, NIBIB/NIH, Faster and Sharper: New Technologies for Visualizing Cells and Embryos
I will discuss our efforts to further develop high resolution optical methods that are better suited for the study of live, dynamic, and 3D samples. Structured illumination microscopy (SIM) doubles the spatial resolution of a light microscope and requires lower light intensities and acquisition times than other super-resolution imaging techniques, but has been almost exclusively applied to the study of single cells. I will discuss a modification of SIM that permits resolution doubling in live embryos and at volumes 5-8x thicker than conventional SIM. I will also describe the application of inverted selective plane illumination microscopy (iSPIM) to the noninvasive study of neurodevelopment in nematode embryos, and our attempts to improve the axial resolution of this technique.
Location: Carl Icahn Lab 101
Date/Time: 11/19/12 at 4:15 pm - 11/19/12 at 5:15 pm
Category: Quantitative & Computational Biology
Department: Lewis-Sigler Institute