Sequencing Core Facility
The Lewis-Sigler Institute for Integrative Genomics houses the Sequencing Core Facility which is operated in conjunction with the Microarray Core Facility and provides researchers with access to state of the art, high-throughput sequencing technologies. The staff provide consultation on experimental design, library preparation, and data analysis. The Sequencing Core Facility works closely with Bioinformatics staff in the Center for Quantitative Biology to provide researchers with computing power and consulting services to analyze sequencing data.
Upgraded Illumina HiSeq 2500 sequencer is mostly running in Rapid-mode now, the run time is 1-3 days with the yield of 100-150 million reads per lane. The Rapid read length is 65 nt or 140 nt, or the exploratory 215 nt. The Rapid flowcell has 2 lanes, which can be loaded with either 1 or 2 library pools.
Please inquire if you still need to run the previous 8-lane flowcells with 1-2 weeks run time.