Sequencing Core Facility
Overview
The Lewis-Sigler Institute for Integrative Genomics houses the Sequencing Core Facility which is operated in conjunction with the Microarray Core Facility and provides researchers with access to state of the art, high-throughput sequencing technologies. The staff provide consultation on experimental design, library preparation, and data analysis. The Sequencing Core Facility works closely with Bioinformatics staff in the Center for Quantitative Biology to provide researchers with computing power and consulting services to analyze sequencing data.
The facility currently houses a state-of-the-art Illumina HiSeq 2000 and two Ion Torrent Personal Genome Machines.
What's New
The new V3 sequencing chemistry is now being used on our Illumina HiSeq 2000 sequencer, producing 120-180 million reads per lane. Keep this in mind when you think about how many libraries to multiplex together to sequence in one lane.
RNA-Seq Workshop - The Lewis-Sigler High Throughput Sequencing Core Facility and Bioinformatics group are providing a hands-on RNA-Seq training session. The session will cover the entire RNA-seq process, from sample collection and preparation all the way through data analysis.
