The Lewis-Sigler High Throughput Sequencing Core Facility and Bioinformatics group are providing a hands-on RNA-Seq training session. The session will cover the entire RNA-seq process, from sample collection and preparation all the way through data analysis. The session is scheduled for April 2-4, 2012 for sample preparation and will be followed by a tutorial on data analysis once the sequencing has been completed.
Faculty, postdocs, graduate students are all welcome to join. There will be a $50 fee charged to cover part of the reagent costs.
Please RSVP to Wei Wang <email@example.com> by Friday, March 30, 2012. Spaces are limited.
April 2, 2012 - 2:00pm to 5:00pm
2:00pm - Introduction to RNA-seq methodology (pdf)
3:00pm - Princeton HTSEQ database (pdf coming soon)
4:00pm - Beyond basics: varieties of specialized RNA-seq (pdf)
April 3 and 4, 2012 - 10:00am to 5:00pm
Hands-on library preparation from total RNA using standard Illumina protocol. Good molecular biology experimental skills are required.
All libraries will be multiplexed and pooled to sequence in 1 lane of 100bp-long reads and sequenced on the HiSeq 2000 over the next few weeks.
A tutorial on sequence read data analysis will be scheduled after the sequencing run is completed.
New session coming soon
Registration for this session is closed, but we are planning another similar workshop soon. Please contact Wei Wang <firstname.lastname@example.org> for more information about how to sign up.