Cornea microstructural and mechanical responses to intraocular pressure
Speaker: Alvin T. Yeh, Texas A&M University
Series: Other Events
Location: J223 Equad
Date/Time: Thursday, October 27, 2011, 10:30 a.m. - 11:30 a.m.
Our present understanding of corneal mechanical responses and underlying structural basis is derived from disparate measurements of tissue biomechanics and ultrastructure performed under different conditions and sample preparations. The accumulation of these measurements has revealed a unique combination of mechanical strength, optical transparency and refractivity that are interrelated to enable the cornea to fulfill its multifunctional role in the eye. For example, cornea mechanical responses initially determine its curvature and refractivity following corrective refractive surgeries. However, prediction of corneal reshaping requires knowledge of cornea mechanical properties that is complicated by its geometry, thickness, nonlinear and heterogeneous mechanical responses. Cornea mechanical responses are intimately related with stromal collagen organization, yet it remains that there is no technology or methodology to both visualize corneal microstructure and measure its mechanical responses in situ. Recent developments in our group will be presented that integrate nonlinear optical microscopy with optical coherence microscopy and enable the simultaneous imaging of collagen microstructure and keratocyte nuclei. Measurements from the central rabbit cornea will be presented that use keratocyte nuclei as fiducial markers for mechanical testing and provide depth-dependent mechanical with microstructural responses.