Quantitative Mass Spectrometry Based Proteomics for Analysis of Eukaryotic Chromatin Structure and Function
We are interested in developing and applying novel mass spectrometry based proteomic methodologies for quantitatively measuring changes in protein expression and protein post-translational modification state of chromatin associated proteins. Specifically, we focus on investigating histone proteins and related histone modifying enzymes and interacting proteins. The overall goal of our work is to investigate the role of histone modifications in gene transcription through a combined use of proteomics, genomics and biochemistry.