I am interested in the role that mechanical forces plays in biology, particularly how nanometer-sized proteins are used to generate cellular-level forces on the micron-scale. Lately, I have been studying how cytoskeletal filaments are used to define intracellular organization, cell shape, mechanics and motility in bacteria. It was recently discovered that bacteria use homologs of the eukaryotic proteins actin and tubulin to guide a number of cellular processes, however physical details of these processes have been lacking due to the minuscule size of most bacterial cells. My lab is developing new experimental techniques that combine mechanical perturbation of cells and molecules with visualization of key protein and macromolecular structures to explore the physical side of these topics. Our toolbox includes unique combinations of optical microscopy, optical trapping, atomic force microscopy, as well as biophysical modeling and simulation.

For more information about my laboratory and research see: http://genomics.princeton.edu/shaevitzlab/

For more information on my teaching and activites see: http://www.princeton.edu/~shaevitz/

1. Deng, Y., Shaevitz, J.W. The effect of aberrations on height calibration in three-dimensional microscopy and particle tracking. Applied Optics 48:1886-1890 (2009). [PDF].
2. Shaevitz, J.W. Bayesian Estimation of the Axial Position in Astigmatism-Based Three-Dimensional Particle Tracking. International Journal of Optics ID:896208 (2009). [PDF].
3. Mignot, T., Shaevitz, J.W. Active and Passive Mechanisms of Intracellular Transport and Localization in Bacteria. Current Opinions in Microbiology 11:580-585 (2008). [PDF]
4. Rosenbluth, M.J., Crow, A., Shaevitz, J.W., Fletcher, D.A. Slow stress propagation in adherent cells. Biophysical Journal 95:6052-6059 (2008). [PDF]
5. Marchesini, S., Boutet, S., Sakdinawat, A.E., Bogan, M.J., Bajt, S., Barty, S., Chapman, H.N., Frank, M., Hau-Riege, S.P., Szoke, A., Cui, A., Shapiro, D.A., Howells, M.R., Spence, J.C.H., Shaevitz, J.W., Lee, J.Y., Hajdu, J., Seibert, M. Massively parallel X-ray holography. Nature Photonics 2:560-563 (2008). [PDF]
6. Shaevitz, J.W., Fletcher, D.A. Curvature and torsion in growing actin networks. Physical Biology 5: 026006 (2008). [PDF]
7. Shaevitz, J.W. Super-resolution for a 3D world, Nature Methods 5:471-472 (2008). [PDF]

For a complete listing see: http://genomics.princeton.edu/shaevitzlab/publications.html