Circular dichroism

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First pioneered by Jean-Baptiste Biot, Augustin Fresnel, and Aimé Cotton [1], circular dichroism (CD) refers to the differential absorption of left and right circularly polarized light.[2][3]. This phenomenon is exhibited in the absorption bands of optically active chiral molecules. CD spectroscopy has a wide range of applications in many different fields. Most notably, UV CD is used to investigate the secondary structure of proteins[4]. UV/Vis CD is used to investigate charge-transfer transitions [5]. Near-infrared CD is used to investigate geometric and electronic structure by probing metal dd transitions[6]. Vibrational circular dichroism, which uses light from the infrared energy region, is used for structural studies of small organic molecules, and most recently proteins and DNA[7].


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