Princeton University Department of Chemistry
US Air Force Office of Scientific Research

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Symposium and Kick-off Meeting of the BioSolarH2 Team
Biological Hydrogen Production from Water and Light:
Current Status & Future Prospects

Friday June 24 – Carl Icahn Lab : Lecture Hall 101
Welcome: Dr. Charles Dismukes, Princeton University
Welcome: Dr. Walter Kozumbo, US Air Force Office of Scientific Research
Dr. Edward Stiefel, session chai
Dr. Ben Hankamer, Univ Queensland, Brisbane, Australia
Photobiological H2 production: Markets, mutants and future potential
Dr. Simon Albracht, University of Amsterdam, The Netherlands
[NiFe]-, [FeFe]- and [Fe]-hydrogenases. What have we learned about their possible application in the Hydrogen Economy?
Dr. Michael Seibert, National Renewable Energy Laboratory, Golden, CO
Biohydrogen Production from Oxygenic Microbes: Issues and Challenges
Dr. Eric Hegg, session chair
11:15 Dr. Maria Ghirardi, National Renewable Energy Laboratory, Golden, CO,
Engineering of a green alga Chlamydomonas r. for improved H2 production
11:50 Dr. Ruediger Schulz , Christian-Albrechts-Universität
Electron transport and hydrogen production in cyanobacteria
12:25 Dr. Oliver Lenz, Humboldt University, Berlin, Germany
Mechanisms of O2 tolerance in [NiFe] hydrogenases from aerobic hydrogen-oxidizing bacteria
1:00 Lunch

Description of the AFOSR MURI Research Project*
Renewable Bio-solar Hydrogen Production from Robust Oxygenic Phototrophs

Dr. Charles Dismukes, Princeton University
Redirecting photosynthetic electron flux into H2 production in cyanobacteria

Dr. Matthew Posewitz, Colorado School of Mines
Screening mutant and native microalgae for improved H2 photoproduction using chemochromic sensors

Dr. Donald Bryant, Penn State University
Cyanobacterial genomics and genetics: approaches to enhanced H2 production

4-5 Round Table Discussion of the AFOSR MURI Project – Room 280

Discussion Leaders:
Dr. Roger Prince – ExxonMobil Research & Engineering Co., Annandale, NJ
Dr. Michael J. Maroney – University of Massachusetts, Amherst, MA
Dr. Ruth Pachter Air Force Research Laboratory, Wright–Patterson AFB, Ohio

5-6 Report Drafting
Saturday June 25 – Carl Icahn Lab – Room 280
9:00 Report Writing
10:00 Round Table Discussion: Presentation of recommendations
11:00 Demonstrations – Hoyt Labs – Room 21

Dr. Gennady Ananyev
-Intracellular redox measurements by Fast-Repetition Rate Fluorometry for H2 uptake & production rate measurements

Dr. Matthew Posewitz
-Chemochromic H2 sensor for screening microbial cultures

2:00 Posters – All posters will be posted on back wall, ground floor of Carl Icahn Lab morning of June 24 (behind Lecture Hall 101)

Dr. Ruth Pachter Air Force Research Laboratory, Wright–Patterson AFB, Ohio
Quantum mechanics/molecular mechanics calculations for an understanding of the catalytic mechanism in Fe-only hydrogenase: outline and preliminary results

Prof. Robert Austin and Dr. Juan Keymer
-Microbial microchemostat

Dr. Derrick Kolling
-Hydrogenase spectroscopy by pulsed EPR

Tyler S. Brown, Dr. Gennady Ananyev, and Prof. Charles Dismukes
- Approaches to ionic and small molecular regulation of hydrogen production in A. maxima

Damian Carrieri
- Sequence and Structure of [NiFe] hydrogenases

Prof. Josh Rabinowitz
-Intracellular metabolomics by mass spectrometry

Drs. Roberto Car and Silviu Zilberman
-DFT computations of electronic structure of [FeFe]-hydrogenase