image database


   

Immunofluorescence database

Database 2 - BrdU-NeuN cells in adult macaque neocortex (red BrdU, green NeuN)

Adult male macaques (Macaques 1-3) - perfused 6 weeks after BrdU injection

To view database 2, first download the following free program: Zeiss LSM Image Browser (this is a Windows based program for PC users). Next, left-click Database 2, which will call up a file download window. Save the compressed database to a local folder on your computer and decompress using WinZip Self-Extractor, StuffIt Expander or other standard software. The folder will then contain a database file (.mdb) and its associated individual .lsm image files. Access the database using Zeiss LSM Image Browser. The browser will allow you to view all z-sections throughout the double-labeled cell (gallery), examine the cells in separate channels (split xy), and measure the size of objects in the image (overlay). Detailed information about the confocal microscope settings used for each scan is included (click the info button).

Orthogonal views

Single channels - NeuN (green), BrdU (red) Notes

 

This BrdU-NeuN cell (arrow) is relatively small and exhibits very little NeuN stained cytoplasm.To view the raw data from this confocal image, download Database 2 and open the file entitled:

"Macaque1BrdUNeuNPFC1"

 

This BrdU-NeuN cell (arrow) is relatively small and has little NeuN stained cytoplasm.Asterisk indicates autofluorescent material which is common in adult primate brain tissue. This profile is distinguishable from stained cells by its shape and staining characteristics. To view the raw data from this confocal image, download database 2 and open the file entitled:

"Macaque1BrdUNeuNPFC2"

This BrdU-NeuN cell (arrow) is relatively small but similar in shape and NeuN staining intensity to some of the NeuN- labeled cells in this field. To view the raw data from this confocal image, download database 2 and open the file entitled:

"Macaque2BrdUNeuNPFC1"

This BrdU-NeuN cell (arrow) is relatively small, lightly stained with NeuN and has no NeuN stained cytoplasm.Asterisk indicates autofluorescent material which is common in adult primate brain tissue. This profile is distinguishable from stained cells by its shape and staining characteristics.To view the raw data from this confocal image, download database 2 and open the file entitled:

"Macaque2BrdUNeuNPFC2"

 

This BrdU-NeuN cell (arrow) is similar in shape to another NeuN-labeled cell in this field.To view the raw data from this confocal image, download Database 2 and open the file entitled:

"Macaque2BrdUNeuNPFC3"

 

 

This BrdU-NeuN cell (arrow) is closely apposed to a NeuN labeled cell which is not BrdU labeled (arrowhead). To view the raw data from this confocal image, download Database 2 and open the file entitled:

"Macaque2BrdUNeuNPFC4"

 

This BrdU-NeuN cell (arrow) is relatively small and is located near two BrdU labeled cells which are not labeled with NeuN. To view the raw data from this confocal image, download Database 2 and open the file entitled:

"Macaque2BrdUNeuNPFC5"

 

This BrdU-NeuN labeled cell (arrow) is closely apposed to a NeuN labeled cell which is not labeled with BrdU. To view the raw data from this confocal image, download Database 2 and open the file entitled:

"Macaque2BrdUNeuNPFC6"

 

This BrdU-NeuN cell (arrow) is small and irregularly shaped. To view the raw data from this confocal image, download Database 2 and open the file entitled:

"Macaque3BrdUNeuNPFC1"