Selected Publications, 1993 - Present
• Brunet AP, Huang ES, Huffine ME, Loeb JE, Weltman RJ & Hecht MH (1993) The Role of Turns in Dictating the Structure of an Alpha-Helical Protein. Nature 364, 355-358. Fig.1-2
• Kamtekar S, Schiffer JM, Xiong H, Babik JM & Hecht MH (1993) Protein Design by Binary Patterning of Polar and Non-Polar Amino Acids. Science 262, 1680-1685. Fig.1-2 Fig.3-4
• Johnson BH & Hecht MH (1994) Recombinant Proteins Can Be Released From E. Coli Cells By Repeated Cycles of Freezing and Thawing. Biotechnology 12, 1357-1360. Fig.1-2 Fig.3-4
• Xiong H, Buckwalter BL, Shieh HM & Hecht MH (1995) Periodicity of Polar and Non-Polar Amino Acids is the Major Determinant of Secondary Structure in Self-Assembling Oligomeric Peptides. Proc. Natl. Acad. Sci. (USA) 92, 6349-6353.
• West MW & Hecht MH (1995) Binary Patterning of Polar and Nonpolar Amino Acids in the Sequences and Structures of Native Proteins. Protein Science 4, 2032-2039.
• Rojas NR, Kamtekar S, Simons CT, McLean JE, Vogel KM, Spiro TG, Farid RS & Hecht MH (1997) De Novo Heme Proteins From Designed Combinatorial Libraries. Protein Science 6, 2512-2524. Fig.1-2 Fig.11
• Rosenbaum DM, Roy S, & Hecht MH (1999) Screening Combinatorial Libraries of De Novo Proteins By Hydrogen-Deuterium Exchange and Electrospray Mass Spectrometry. J. Am. Chem. Soc. 121, 9509-9513.
• West MW, Wang W, Patterson J, Mancias JD, Beasley JR & Hecht MH (1999) De Novo Amyloid Proteins From Designed Combinatorial Libraries. Proc. Natl. Acad. Sci. (USA) 96, 11211-11216.
• Broome BM & Hecht MH (2000) Nature Disfavors Sequences of Alternating Polar and Nonpolar Amino Acids: Implications for Amyloidogenesis. J. Molecular Biology 296, 961-968.
• Roy S & Hecht MH (2000) Cooperative Thermal Denaturation of Proteins Designed by Binary Patterning of Polar and Nonpolar Amino Acids. Biochemistry 39, 4603-4607.
• Moffet DA, Certain LK, Smith AJ, Kessel AJ, Beckwith KA &Hecht MH (2000) Peroxidase Activity in Heme Proteins Derived From a Designed Combinatorial Library. J. Am. Chem. Soc. 122, 7612-7613.
• Moffet DA, Case MA, House JC, Vogel K, Williams R, Spiro TG, McLendon GL, Hecht MH (2001) Carbon Monoxide Binding by De Novo Heme Proteins From a Designed Combinatorial Library. J. Am. Chem. Soc. 123, 2109-2115.
• Xu, G, Wang W, Groves JT, Hecht MH (2001) Self-Assembled Monolayers from a Designed Combinatorial Library of De Novo Beta-Sheet Proteins. Proc. Natl. Acad. Sci. (USA)98, 3652-3657.
• Moffet DA & Hecht MH (2001) De Novo Proteins From Combinatorial Libraries. Chemical Reviews 101, 3191-3204.
• Wang W, & Hecht MH (2002) Rationally Designed Mutations Convert De Novo Amyloid-Like Fibrils into Soluble Monomeric Beta-Sheet Proteins. Proc. Natl. Acad. Sci. (USA) 99, 2760-2765.
• Wurth C, Guimard NK, & Hecht MH. (2002) Mutations that Reduce Aggregation of the Alzheimer’s A-Beta42 Peptide: An Unbiased Search for the Sequence Determinants of A-Beta Amyloidogenesis. J. Molec. Biology 319, 1279-1290.
• Brown CL, Aksay IA, Saville DA, & Hecht MH (2002) Template-Directed Assembly of a De Novo Designed Protein. J. Am. Chem. Soc. 124, 6846-6848.
• Wei Y, Liu T, Sazinsky SL, Moffet DA, Pelczer I, & Hecht MH (2003) Stably Folded De Novo Proteins From a Designed Combinatorial Library. Protein Science 12, 92-102.
• Moffet DA, Foley J, & Hecht MH (2003) Midpoint Reduction Potentials and Heme Binding Stoichiometries of De Novo Proteins from Designed Combinatorial Libraries. Biophysical Chemistry 105, 231-239.
• Wei Y, Kim S, Fela D, Baum J, & Hecht MH (2003) Solution Structure of a De Novo Protein From a Designed Combinatorial Library. Proc. Natl. Acad. Sci. (USA) 100, 13270-13273.
• Wei Y & Hecht MH (2004) Enzyme-like Proteins From an Unselected Library of Designed Amino Acid Sequences. Protein Engineering, Design and Selection 17, 67-75.
• Hecht MH, Das A, Go A, Bradley LH, Wei Y (2004) De Novo Proteins From Designed Combinatorial Libraries. Protein Science 13, 1711-1723.
• Klepeis JL, Wei Y, Hecht MH & Floudas CA (2004) Ab Initio Prediction of the 3-Dimensional Structure of a De Novo Designed Protein: A Double Blind Case Study. Proteins: Structure, Function, and Bioinformatics 58, 560-570.
• Bradley LH, Kleiner RE, Wang AF, Hecht MH & Wood DW (2005) An Intein-Based Genetic Selection Enables Construction of a High-Quality Library of Binary Patterned De Novo Sequences. Protein Engineering, Design and Selection 18, 201-207.
• Hu Y, Das A, Hecht MH & Scoles G (2005) Nanografting De Novo Proteins onto Gold Surfaces. Langmuir 21,9103-9109.
• Kim W & Hecht MH (2005) Mutagenesis of the Carboxy-Terminal Residues of the Alzheimer’s Peptide: Sequence Determinants of Enhanced Amyloidogenicity of A-Beta42 Relative to A-Beta40. J. Biological Chemistry 280, 35069-35076.
• Bradley LH, Thumfort P, Hecht MH. (2006) De Novo Proteins from Binary Patterned Combinatorial Libraries. Chapter 3 in Protein Design: Methods & Applications in Methods in Molecular Biology (Humana Press) 340, 53-69.
• Das A, Trammell SA, & Hecht MH (2006) Electrochemical and ligand binding studies of a de novo heme protein. Biophysical Chemistry 123,102-112.
• Wurth C, Kim W, & Hecht MH (2006) Combinatorial Approaches to Probe the Sequence Determinants of Protein Aggregation and Amyloidogenesis. Protein and Peptide Letters 13, 279-286.
• Kim W, Kim Y, Min J, Kim DJ, Chang YT, & Hecht MH (2006) A High-Throughput Screen for Compounds That Inhibit Aggregation of the Alzheimer’s Peptide. ACS Chemical Biology 1, 461-469.
• Kim W & Hecht MH (2006) Generic hydrophobic residues are sufficient to promote aggregation of the Alzheimer's Abeta42 peptide. Proc. Natl. Acad. Sci. 103, 15824-15829.
• Das A, Hecht MH (2007) Peroxidase Activity of De Novo Heme Proteins Immobilized on Electrodess. J. Inorganic Biochemistry 101, 1820-1826.
• Kim W, Hecht MH (2008) Mutations Enhance the Aggregation Propensity of the Alzheimer’s Ab Peptide. J. Molec. Biology 377, 565-574.
• Go A, Kim S, Baum J, & Hecht MH (2008) Structure and Dynamics of De novo Proteins from a Designed Superfamily of 4-Helix Bundles. Protein Science 17, 4.
