Princeton University         Office of Technology & Trademark Licensing
                                                Fourth Floor, New South Building
                                                Post Office Box 36
                                                Princeton, New Jersey 08544-0036
                                                Phone: (609) 258-1570
                                                FAX: (609) 258-1159

 

Two New Strains Of E. Coli For Use In Recombinant DNA Techniques

Researchers at Princeton University have developed two new strains of E. coli for working with BAC (bacterial artificial chromosome) plasmid DNA. Princeton is currently seeking industrial collaborators to commercialize this technology.

Both new strains (GS237 and GS243) are isogenic with DH10B but carry a modified allele which improves transformation efficiency 5-fold. The new genotype benefits the transformation of both BAC plasmids and standard cloning plasmids such as pUC19, and once applied should benefit additional cloning strains including AG1, DH1, DH5a, JM109, JS4, Top10, and XL1-blue.

In addition, strain GS243 carries a repaired recA+ allele (replacing the recA1 allele of DH10B). By producing wild-type RecA protein in the modified DH10B background, strain GS243 simplifies the application of traditional genetic recombination methods to BAC plasmids. The stability of BAC DNA in GS243 is similar to that seen with DH10B, allowing targeted recombination with BAC plasmids without inadvertent loss or rearrangement of the BAC construct. The recA+ allele of GS243 further improves BAC transformation, resulting in efficiencies typically 100-fold greater than DH10B.

For more information please contact:
             William H. Gowen
             Office of Patents and Licensing
             Princeton University
             4 New South Building
             Princeton, NJ 08544-0036
             (609) 258-6762
             (609) 258-1159 fax
             wgowen@princeton.edu

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