Computers and Software Technologies
Princeton University
Office of Technology Licensing and Intellectual Property
NEW USES OF THE Cre/Lox RECOMBINATION SYSTEM AND THE PSEUDORABIES VIRUS NEURAL CIRCUIT TRACING SYSTEM
Researchers at Princeton University have developed a novel method for tracing the neural circuits of the central nervous system (CNS). Princeton is currently seeking industrial collaborators to commercialize this technology.
Traditionally, tracing the neural circuits of the CNS has been limited by the lack of a suitable method for trans-synaptic tracing. This new method has the capability of retrograde tracing of specific neural connections across multiple synapses. This is accomplished by using a recombinant alpha-herpesvirus (pseudorabies virus; PRV) which is dependent on a Cre-mediated recombination event for expression of the essential tk gene and green fluorescent protein. By using the virus and this concept, researchers have the potential to trace any neural circuit in an animal where transgenic strains can be constructed with any specific recombinase expressed in neurons.
It is anticipated that this new technology can be used in the precise delineation of the neural circuits that respond to varying factors or stimuli, and can lead to a more complete understanding of the mechanisms responsible for the regulation of human behavior.
Patent protection is pending.
For more information please contact:
William H. Gowen
Office of Technology Licensing and Intellectual Property
Princeton University
4 New South Building
Princeton, NJ 08544-0036
(609) 258-6762
(609) 258-1159 fax
wgowen@princeton.edu
Computers and Software Technologies
Back to Available Technologies