Xanthine oxidase

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Xanthine oxidase (XO (sometimes 'XAO'), a form of xanthine oxidoreductase that generates reactive oxygen species[2]) is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. This enzyme plays an important role in the catabolism of purines in some species, including humans.[3][4]

Xanthine oxidase can be converted to xanthine dehydrogenase by reversible sulfhydryl oxidation.[5]

Contents

Reaction

The following chemical reactions are catalyzed by xanthine oxidase:

  • hypoxanthine + H2O + O2 \rightleftharpoons xanthine + H2O2
  • xanthine + H2O + O2 \rightleftharpoons uric acid + H2O2

hypoxanthine (one oxygen atom)

xanthine (two oxygens)

uric acid (three oxygens)

Protein structure

The protein is large, having a molecular weight of 270,000, and has 2 flavin molecules (bound as FAD), 2 molybdenum atoms, and 8 iron atoms bound per enzymatic unit. The molybdenum atoms are contained as molybdopterin cofactors and are the active sites of the enzyme. The iron atoms are part of [2Fe-2S] ferredoxin iron-sulfur clusters and participate in electron transfer reactions.

Catalytic mechanism

The active site of XO is composed of a molybdopterin unit with the molybdenum atom also coordinated by terminal oxygen (oxo), sulfur atoms and a terminal hydroxide.[6] In the reaction with xanthine to form uric acid, an oxygen atom is transferred from molybdenum to xanthine, whereby several intermediates are assumed to be involved. [7] The reformation of the active molybdenum center occurs by the addition of water. Like other known molybdenum-containing oxidoreductases, the oxygen atom introduced to the substrate by XO originates from water rather than from dioxygen (O2).

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