MOL 101 / STC 101

From DNA to Human Complexity

Professor/Instructor

Bonnie Lynn Bassler, Eric Francis Wieschaus, Heather A. Thieringer

This lecture and laboratory course will acquaint non-biology majors with the theory and practice of modern molecular biology, with a focus on biological topics of current public interest. Topics include: structure of DNA, RNA, proteins, genomes and an overview of state-of-the-art technologies including cloning, recombinant DNA and PCR. The course will address how recent scientific advances affect issues relevant to human biology including forensics, stem cells, molecular evolution and the genetic basis of human traits and behaviors such as obesity and aggression. Three lectures, one three-hour laboratory.

NEU 101 / MOL 110 / STC 102

Neuroscience and Everyday Life

Professor/Instructor

Samuel Sheng-Hung Wang, Alan Gelperin

Acquaints non-science majors with classical and modern neuroscience. Lectures will give an overview at levels ranging from molecular signaling to cognitive science with a focus on the neuroscience of everyday life, from the general (love, memory, and personality) to the particular (jet lag, autism, and weight loss). The laboratory will offer hands-on experience in recording signals from single neurons, examining neural structures, and analysis of whole-brain functional brain imaging data. Two 90-minute lectures, one laboratory.

EEB 211 / MOL 211

Life on Earth: Chaos and Clockwork of Biological Design

Professor/Instructor

Daniel Ian Rubenstein, Andrew P. Dobson, Joshua Akey

An examination of how life evolved and how organisms function. Design--'intelligent' and otherwise--will provide a unifying theme. Why do some microbes produce slime and others do not? Why are males brightly colored in some species, but in others females are the showy sex? Why do humans have knees that fail whereas horses and zebras do not? These and other 'why is it so' questions related to the origin and history of life, genetic code, biochemistry, physiology, morphology and body plans, sex and reproduction, cooperation, and ecosystems will be explored. This course is required of all EEB majors and fulfills a requirement for medical school.

MOL 214 / EEB 214

Introduction to Cellular and Molecular Biology

Professor/Instructor

Important concepts and elements of molecular biology, biochemistry, genetics, and cell biology, are examined in an experimental context. This course fulfills the requirement for students majoring in the biological sciences and satisfies the biology requirement for entrance into medical school. Two 90-minute lectures, one three-hour laboratory.

MOL 215 / EEB 215 / CBE 215

Quantitative Principles in Cell and Molecular Biology

Professor/Instructor

Alexei V. Korennykh, Jared E. Toettcher, Philip George Felton

Central concepts and experiments in cellular, molecular, and developmental biology with an emphasis on underlying physical and engineering principles. Topics include the genetic code; energetics and cellular organization; communication, feeding, and signaling between cells; feedback loops and cellular organization; problems and solutions in development; the organization of large cellular systems, such as the nervous and immune systems. Satisfies the biology requirement for entrance into medical school. Prerequisites: AP biology, physics, and calculus. Three lectures, one three-hour laboratory.

ISC 231 / CHM 231 / COS 231 / MOL 231 / PHY 231

An Integrated, Quantitative Introduction to the Natural Sciences I

Professor/Instructor

Curtis Gove Callan Jr., Joshua William Shaevitz, Peter Andolfatto

An integrated, mathematically and computationally sophisticated introduction to physics, chemistry, molecular biology, and computer science. Alternative to the combination of PHY 103-104, CHM 201-202, MOL 214-215 and COS126. Students must enroll in ISC231 and ISC232 in the fall and ISC233 and ISC234 in the spring. Prerequisites: familiarity with calculus at the level of MAT103/104 or Advanced Placement Calculus BC, solid high school physics and chemistry courses. Five lectures, one three-hour laboratory, one three-hour computational laboratory, one evening problem session.

ISC 232 / CHM 232 / COS 232 / MOL 232 / PHY 232

An Integrated, Quantitative Introduction to the Natural Sciences I

Professor/Instructor

Peter Andolfatto, Curtis Gove Callan Jr., Joshua William Shaevitz

An integrated, mathematically and computationally sophisticated introduction to physics, chemistry, molecular biology, and computer science. Alternative to the combination of PHY 103-104, CHM 201-202, MOL 214-215 and COS126. Students must enroll in ISC231 and ISC232 in the fall and ISC233 and ISC234 in the spring. Prerequisites: familiarity with the calculus at the level of MAT 103-104 or Advanced Placement Calculus BC, solid high school physics and chemistry courses. Five lectures, one three-hour laboratory, one three-hour computational laboratory, one evening problem session.

ISC 233 / CHM 233 / COS 233 / MOL 233 / PHY 233

An Integrated, Quantitative Introduction to the Natural Sciences II

Professor/Instructor

Joshua William Shaevitz, Olga G. Troyanskaya, Haw Yang

An integrated, mathematically and computationally sophisticated introduction to physics and chemistry, drawing on examples from biological systems. Alternative to the combination of PHY 103-104, CHM 201-202, MOL 214-215, and COS126. Students must enroll in ISC231 and ISC232 in the fall and ISC233 and ISC234 in the spring. Prerequisites: familiarity with the calculus at the level of MAT 103-104 or Advanced Placement Calculus BC, solid high school physics and chemistry courses. Five lectures, one three-hour laboratory, one three-hour computational laboratory, one evening problem session.

ISC 234 / CHM 234 / COS 234 / MOL 234 / PHY 234

An Integrated, Quantitative Introduction to the Natural Sciences II

Professor/Instructor

Joshua William Shaevitz, Olga G. Troyanskaya, Haw Yang

An integrated, mathematically and computationally sophisticated introduction to physics and chemistry, drawing on examples from biological systems. Alternative to the combination of PHY 103-104, CHM 201-202, MOL 214-215 and COS126. Students must enroll in ISC231 and ISC232 in the fall and ISC233 and ISC234 in the spring. Prerequisites: familiarity with the calculus at the level of MAT 103-104 or Advanced Placement Calculus BC, solid high school physics and chemistry courses. Five lectures, one three-hour laboratory, one three-hour computational laboratory, one evening problem session.

EEB 327 / MOL 327 / GHP 327

Immune Systems: From Molecules to Populations

Professor/Instructor

Andrea Linn Graham

Why is there immunological polymorphism in animal populations? Why do immune systems work as they do? This course examines the theories of host-parasite coevolution, including optimal host resource allocation to immune defense in light of parasite counter-strategies, and assesses the empirical evidence by which these theories are tested. Students look at the evolutionary ecology of mechanisms used by immune systems to recognize and kill parasites, finding similarities across animal taxa. Finally, students will map immune mechanisms onto host phylogenies to understand the order in which different mechanisms arose over evolutionary time.

EEB 320 / MOL 330

Molecular Evolution

Professor/Instructor

Laura Faye Landweber

How and where did life evolve? This advanced seminar will discuss the evolution of the molecules that sustain life (DNA, RNA and proteins) at both the micro and macro evolutionary levels. We will explore the role of these molecules in the origin and continued evolution of life. Topics include the origin of eukaryotes and organelles, comparative genomics, population genetics, the microbiome, and human evolution. Prerequisites: 214, 215, or CHM 236. One three-hour seminar. Note that students new to either evolution or genetics will find 309 more appropriate.

MOL 340

Molecular and Cellular Immunology

Professor/Instructor

Alexander Ploss

A broad survey of the field of immunology and the mammalian immune system. The cellular and molecular basis of innate and acquired immunity will be discussed in detail. The course will provide frequent exemplars drawn from human biology in health and disease. Prerequisite: MOL214/215.

MOL 342

Genetics

Professor/Instructor

Mohamed S. Abou Donia, Gertrud Maria Schupbach, Michael Steven Levine

Basic principles of genetics illustrated with examples from prokaryote and eukaryote organisms with emphasis on classic genetic techniques. The evolving conception of the gene and genome will be the primary focus of the course. Selected advanced topics will include Drosophila developmental genetics, yeast cell biology, and human disease. Two 90-minute lectures, one class. Prerequisite: MOL 214/215, or permission of instructor.

MOL 345 / CHM 345

Biochemistry

Professor/Instructor

Fundamental concepts of biomolecular structure and function will be discussed, with an emphasis on principles of thermodynamics, binding and catalysis. A major portion of the course will focus on metabolism and its logic and regulation. Prerequisites: MOL 214/215 and either CHM 304/304B or ISC 335. CHM 304/304B may be taken concurrently with MOL 345.

MOL 348

Cell and Developmental Biology

Professor/Instructor

Rebecca D. Burdine, Danelle Devenport

The mechanisms that underlie development of multicellular organisms, from C. elegans to humans, will be examined using biochemical, genetic and cell biological approaches. The course will investigate the roles that gene regulation, cell-cell communication, cell adhesion, cell motility, cell death, signal transduction and intracellular trafficking play in the commitment, differentiation and assembly of cells into specialized tissues. Two 90-minute lectures, one two-hour class. Prerequisite: MOL 214/215.

MOL 350

Laboratory in Molecular Biology

Professor/Instructor

Bruce Patterson, Liz Wright

The major objective of the course is to introduce students to a variety of tools required to perform independent research in the field of molecular biology. While conducting original research, students will employ a number of techniques that are used by molecular biologists, molecular geneticists, and biochemists. Students will gain an understanding of how, when, and why certain techniques and skills are used in a research setting. In addition, students will learn to write a research report modeled on the scientific literature. One lecture, two three-hour laboratories. Prerequisite: MOL 214/215.

EEB 355 / MOL 355

Introduction to Statistics for Biology

Professor/Instructor

John D. Storey, Peter Andolfatto

An applied introduction to probability and statistical methods in biology. Topics include data visualization, parameter estimation, frequentist hypothesis testing, Bayesian hypothesis testing, useful statistical tests, linear models, and an introduction to data-driven modeling. Analyses will use an appropriate statistical computer package.

NEU 408 / MOL 408 / PSY 404

Cellular and Systems Neuroscience

Professor/Instructor

Timothy J. Buschman, Ilana Basya Witten

A survey of fundamental principles in neurobiology at the biophysical, cellular, and system levels. Lectures will address the basis of the action potential, synaptic transmission and plasticity, local circuit computation, sensory physiology, and motor control. Prerequisites: MOL 214 or MOL 215, PSY 258, PHY 103-104, and MAT 103-104, or permission of instructor. Two 90-minute lectures, one preceptorial.

MOL 410

Introduction to Biological Dynamics

Professor/Instructor

Ned S. Wingreen, Thomas Gregor

Designed for students in the biological sciences, this course focuses on the application of mathematical methods to biological problems. Intended to provide a basic grounding in mathematical modeling and data analysis for students who might not have pursued further study in mathematics. Topics include differential equations, linear algebra, difference equations, and probability. Each topic will have a lecture component and computer laboratory component. Students will work extensively with the computing package MATLAB. No previous computing experience necessary. Two 90-minute lectures, one laboratory.

EEB 414 / MOL 414

Genetics of Human Populations

Professor/Instructor

Peter Andolfatto

This seminar surveys the evolutionary history of modern humans and the genetic basis of variation in our species through reading and discussion of classic and contemporary primary literature. Topics include the evolutionary origins of modern human populations, signatures of natural selection in the human genome, and approaches for discovering genetic variants that affect disease susceptibility and variation in normal traits. Significant emphasis is placed on recent advances made possible by the human genome project. Prerequisites: 309, or 320, or MOL 342, or CHM 236. One three-hour seminar.

MOL 425 / WWS 355 / GHP 425

Infection: Biology, Burden, Policy

Professor/Instructor

Adel A. F. Mahmoud, Thomas Eugene Shenk

This course will examine fundamental determinants of human microbe interaction at the biological and ecological levels. The focus will be on major global infectious diseases, their burden of illness and policy challenges for adequate prevention and control. Each infectious agent will be discussed in terms of its biology, mechanisms of pathogenesis, and epidemiology, as well as strategies for its control. Specific emphasis will be placed on the public health aspects of each disease. Prerequisite: MOL 101/214/215, or permission of instructor. Two 90-minute lectures.

MOL 433 / CBE 434 / GHP 433

Biotechnology

Professor/Instructor

Jane Flint

This course will consider the principles, development, outcomes and future directions of therapeutic application of biotechnology, with particular emphasis on the interplay between basic research and clinical experience. Topics to be discussed include production of hormones and other therapeutic proteins, gene therapy, oncolytic viruses, and stem cells. Reading will be from the primary literature. Prerequisite: MOL 214/215.

MOL 435

Pathogenesis and Bacterial Diversity

Professor/Instructor

An examination of current topics exploring the microbial world with emphasis on signal transduction, and the molecular basis for bacterial diversity and their roles in bacterial pathogenesis. Topics will include the regulation of cell division and sporulation, quorum sensing, mechanisms of microbial differentiation, evolution of communicable diseases, molecular mechanisms of pathogenesis, and identification of virulence factor and immunization. Two lectures, one precept. Prerequisites: MOL214/215, or permission of instructor.

NEU 437 / MOL 437 / PSY 437

Computational Neuroscience

Professor/Instructor

Carlos D. Brody

Introduction to the biophysics of nerve cells and synapses, and the mathematics of neural networks. How can networks of neurons compute? How do we model and analyze data from neuroscientific experiments? Data from experiments running at Princeton will be used as examples (e.g., blowfly visual system, hippocampal slice, rodent prefrontal cortex). Each topic will have a lecture and a computer laboratory component. Prerequisite: MOL 410, or elementary knowledge of linear algebra, differential equations, probability, and basic programming ability, or permission of the instructor. Two 90 minute lectures, one laboratory.

CBE 438 / MOL 438

Biomolecular Engineering

Professor/Instructor

A. James Link

This course will focus on the design and engineering of biomacromolecules. After a brief review of protein and nucleic acid chemistry and structure, we will delve into rational, evolutionary, and computational methods for the design of these molecules. Specific topics to be covered include aptamers, protein and RNA-based switches and sensors, unnatural amino acids and nucleotides, enzyme engineering, and the integration of these parts via synthetic biology efforts. Three lectures.